Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Abbassia, Cairo, P.O. Box 11381, Egypt.
Mol Biol Rep. 2019 Aug;46(4):4581-4590. doi: 10.1007/s11033-019-04810-x. Epub 2019 Apr 19.
Recent trends are moving towards the use of the circulating transcriptome as a potential diagnostic and therapeutic tool for hepatocellular carcinoma (HCC). The aim of this study is to identify circulatory RNA based biomarker panel, in addition to their relationship to the outcome in HCC. First, utilizing bioinformatics tools, we selected an HCC-specific RNA-based biomarker panel that depended on the integration of suppressor of glucose autophagy-associated (SOGA1) gene expression with the chosen panel of epigenetic regulators of this gene [long non-coding RNA antisense for X-inactive-specific transcript (lncRNA-TSIX) and microRNA-548-a-3p (miR-548-a-3p)]. Second, we attempted to validate these biomarkers using the sera of 65 patients with HCC, 34 patients with chronic hepatitis C virus (CHC) infection and 32 healthy volunteers. Finally, the expression levels of the chosen RNA-based biomarker panel were assessed in the serum samples using qRT-PCR assays. The panel of 3 RNA-based biomarkers (lncRNA-TSIX, miR-548-a-3p, and SOGA1) exhibited high sensitivity and specificity in differentiating HCC patients from CHC patients and healthy controls. Among these 3 RNAs, serum lncRNA-TSIX and SOGA1 were independent prognostic factor. The chosen circulatory RNA-based biomarker panel may serve as a diagnostic and prognostic biomarker for HCC.
最近的趋势是将循环转录组作为肝细胞癌（HCC）的潜在诊断和治疗工具。本研究旨在确定基于循环 RNA 的生物标志物谱，以及它们与 HCC 结局的关系。首先，利用生物信息学工具，我们选择了一个基于 HCC 特异性 RNA 的生物标志物谱，该谱依赖于抑制葡萄糖自噬相关（SOGA1）基因表达与所选基因的表观遗传调节剂（长链非编码 RNA 反义 X 失活特异性转录物（lncRNA-TSIX）和 microRNA-548-a-3p（miR-548-a-3p））的整合。其次，我们试图使用 65 例 HCC 患者、34 例慢性丙型肝炎病毒（CHC）感染患者和 32 名健康志愿者的血清来验证这些生物标志物。最后，使用 qRT-PCR 检测血清样本中所选基于 RNA 的生物标志物谱的表达水平。该 3 个基于 RNA 的生物标志物（lncRNA-TSIX、miR-548-a-3p 和 SOGA1）的组合在区分 HCC 患者与 CHC 患者和健康对照者方面具有较高的敏感性和特异性。在这 3 种 RNA 中，血清 lncRNA-TSIX 和 SOGA1 是独立的预后因素。所选的循环 RNA 基于生物标志物的谱可能作为 HCC 的诊断和预后生物标志物。
