Department of Brain and Cognitive Sciences, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran.
J Cell Physiol. 2019 Nov;234(11):20742-20754. doi: 10.1002/jcp.28680. Epub 2019 Apr 19.
A large number of treatment approaches have been used for spinal cord injury improvement, a medically incurable disorder, and subsequently stem cell transplantation appears to be a promising strategy. The main objective of this study is to ascertain whether combinational therapy of human neural stem cells (hNSCs) together with lithium chloride improves cell survival, proliferation, and differentiation in a rat spinal contusion model, or not. Contusive spinal cord injury was implemented on Wistar male rats. Experimental groups comprised of: control, hNSCs transplanted, lithium chloride (Li), and hNSCs and lithium chloride (hNSCs + Li). In every experimental group, locomotor activity score and motor evoked potential (MEP) were performed to evaluate motor recovery as well as histological assessments to determine mechanisms of improvement. In accordance with our results, the hNSCs + Li and the Li groups showed significant improvement in locomotor scores and MEP. Also, Histological assessments revealed that transplanted hNSCs are capable of differentiation and migration along the spinal cord. Although NESTIN-positive cells were proliferated significantly in the Lithium group in comparison with control and the hNSCs + Li groups, the quantity of ED1 cells in the hNSCs + Li was significantly larger than the other two groups. Our results demonstrate that combinational therapy of hNSCs with lithium chloride and lithium chloride individually are adequate for ameliorating more than partial functional recovery and endogenous repair in spinal cord-injured rats.
大量的治疗方法已被用于改善脊髓损伤，这是一种医学上无法治愈的疾病，随后干细胞移植似乎是一种很有前途的策略。本研究的主要目的是确定人神经干细胞（hNSCs）与氯化锂联合治疗是否能提高大鼠脊髓挫伤模型中的细胞存活率、增殖和分化。在 Wistar 雄性大鼠中实施挫伤性脊髓损伤。实验组包括：对照组、hNSCs 移植组、氯化锂（Li）组和 hNSCs 和氯化锂（hNSCs+Li）组。在每个实验组中，进行运动活动评分和运动诱发电位（MEP）以评估运动恢复情况，以及进行组织学评估以确定改善机制。根据我们的结果，hNSCs+Li 和 Li 组在运动评分和 MEP 方面均有显著改善。此外，组织学评估显示，移植的 hNSCs 能够沿着脊髓分化和迁移。虽然与对照组和 hNSCs+Li 组相比，锂组中 NESTIN 阳性细胞的增殖显著增加，但 hNSCs+Li 组中的 ED1 细胞数量明显大于其他两组。我们的结果表明，hNSCs 与氯化锂联合治疗以及单独使用氯化锂均可改善脊髓损伤大鼠的部分功能恢复和内源性修复。
