IPCB/CNR, Institute of Polymers, Composites and Biomaterials - Consiglio Nazionale delle Ricerche, Mostra D'Oltremare, Pad. 20, V.le J.F. Kennedy 54, 80125, Naples, Italy.
Department of Chemical, Materials and Industrial Production Engineering, University of Naples Federico II, Naples, Italy.
J Biomed Mater Res A. 2019 Aug;107(8):1803-1813. doi: 10.1002/jbm.a.36699. Epub 2019 Apr 29.
The use of bioactive proteins such as keratin has been successfully explored to improve the biological interface of scaffolds with cells during the tissue regeneration. In this work, it is optimized the fabrication of nanofibers combining wool keratin extracted by sulfitolysis, with polycaprolactone (PCL) in order to design bicomponent fibrous matrices able to exert a self-adapting pattern of signals-morphological, chemical, or physical-confined at the single fiber level, to influence cell and bacteria interactions. It is demonstrated that the blending of highly polydisperse keratin with PCL (50:50) improves the stability of the electrospinning process, promoting the formation of nanofibers-144.1 ± 43.9 nm-without the formation of defects (i.e., beads, ribbons) typically recognized in the fabrication of keratin ones. Moreover, keratin drastically increases the fiber hydrophilicity-compared with PCL fiber alone-thus improving the hMSC adhesion and in vitro proliferation until 14 days. Moreover, the growth of bacterial strains (i.e., Escherichia coli and Staphylococcus aureus) seems to be not specifically inhibited by the contribution of keratin, so that the integration of further selected compounds (i.e., metal ions) is suggested to more efficiently fight against bacteria resistance, to make them suitable for the regeneration of different interfaces and soft tissues (i.e., skin and cornea). © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1803-1813, 2019.
利用生物活性蛋白（如角蛋白）已成功探索用于改善组织再生过程中支架与细胞的生物界面。在这项工作中，通过亚硫酸氢盐法提取羊毛角蛋白，并与聚己内酯（PCL）相结合，优化了纳米纤维的制备，以设计能够在单纤维水平上发挥信号形态、化学或物理限制的自适应模式的双组分纤维基质，从而影响细胞和细菌的相互作用。结果表明，将高度多分散的角蛋白与 PCL（50:50）混合可提高电纺过程的稳定性，促进纳米纤维的形成（144.1±43.9nm），而不会形成通常在角蛋白制备过程中发现的缺陷（即珠粒、带状物）。此外，角蛋白可极大地提高纤维的亲水性-与单独的 PCL 纤维相比-从而提高 hMSC 的黏附和体外增殖能力，直到 14 天。此外，细菌菌株（即大肠杆菌和金黄色葡萄球菌）的生长似乎不是由于角蛋白的贡献而被特异性抑制，因此建议整合进一步选择的化合物（即金属离子），以更有效地对抗细菌耐药性，使其适合不同界面和软组织（即皮肤和角膜）的再生。©2019 年 Wiley 期刊出版公司。J 生物医学材料研究 A 部分：107A：1803-1813，2019 年。
