Bie Zijun, Huang Ailan, Zhang Yanan, Chen Yang
School of Pharmacy, Bengbu Medical University, 2600 Donghai Avenue, Bengbu, 233000, China; Department of Chemistry, Bengbu Medical University, 2600 Donghai Avenue, Bengbu, 233030, China.
Department of Chemistry, Bengbu Medical University, 2600 Donghai Avenue, Bengbu, 233030, China.
Anal Chim Acta. 2019 Aug 13;1065:40-48. doi: 10.1016/j.aca.2019.03.034. Epub 2019 Mar 21.
Development of novel matrix for surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) is a hot-spot in research frontiers. Herein, we established novel SALDI-MS approach for the analysis of cis-diol small molecules by using boronate affinity metal-organic frameworks (BA-MOFs) as the sorbent for extraction and later as matrix for SALDI. In the meantime, the prepared BA-MOFs exhibited several significant merits, such as excellent selectivity, tolerance to interference and fast binding equilibria. By integrating the advantages of MOFs and the molecular recognition of boronate affinity, a novel platform for simultaneous enrichment and MS detection of cis-diol small molecules was proposed for the first time. There are only two steps in the operation procedure, which greatly simplified the whole experimental workflow. Efficient analysis of cis-diol small molecules in real samples were also well demonstrated. This proposed novel protocol not only gave new insights into the application of functionalized MOFs, but also promoted the development of SALDI- MS.
开发用于表面辅助激光解吸/电离质谱（SALDI-MS）的新型基质是研究前沿的一个热点。在此，我们建立了一种新型的SALDI-MS方法，用于分析顺式二醇小分子，该方法使用硼酸酯亲和金属有机框架（BA-MOFs）作为萃取吸附剂，随后作为SALDI的基质。同时，制备的BA-MOFs表现出几个显著优点，如优异的选择性、抗干扰能力和快速的结合平衡。通过整合金属有机框架的优势和硼酸酯亲和的分子识别能力，首次提出了一个用于顺式二醇小分子同时富集和质谱检测的新型平台。操作过程仅需两步，极大地简化了整个实验流程。该方法也很好地证明了对实际样品中顺式二醇小分子的高效分析。所提出的这一新型方案不仅为功能化金属有机框架的应用提供了新的见解, 也推动了SALDI-MS的发展。
