Departamento de Morfología y Biología Celular, Grupo SINPOS Universidad de Oviedo, Spain.
Departamento de Morfología y Biología Celular, Grupo SINPOS Universidad de Oviedo, Spain; Servicio de Anatomía Patológica, Hospital Universitario de Salamanca, Salamanca, Spain.
Ann Anat. 2019 Jul;224:62-72. doi: 10.1016/j.aanat.2019.02.007. Epub 2019 Apr 18.
Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule). The cells forming those compartments are continuous and share the properties of that covering the nerve fibers. Small leucine-rich proteoglycans are major proteoglycans of the extracellular matrix and regulate collagen fibrillogenesis, cell signalling pathways and extracellular matrix assembly. Here we used immunohistochemistry to investigate the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles. The distribution of these compounds was: the inner core express decorin, biglycan, lumican, fibromodulin, osteoadherin; the intermediate layer display immunoreactivity for osteoadherin; the outer core biglycan, decorin, lumican, fibromodulin and osteoadherin; and the capsule contains biglycan, decorin, fibromodulin, and lumican. Asporin, prolargin and keratocan were undetectable. These results complement our knowledge about the distribution of small leucine-rich proteoglycans in human Pacinian corpuscles, and help to understand the composition of the extracellular matrix in these sensory formations.
帕西尼小体是呈洋葱状的多层机械感受器，由轴突末梢、雪旺氏相关细胞（内芯）、神经内膜相关细胞（中间层）和神经外膜相关细胞（外芯-囊）组成。形成这些隔室的细胞是连续的，并具有覆盖神经纤维的特性。小富含亮氨酸的蛋白聚糖是细胞外基质的主要蛋白聚糖，调节胶原纤维的形成、细胞信号通路和细胞外基质的组装。在这里，我们使用免疫组织化学方法研究了人类皮肤帕西尼小体中小富含亮氨酸的蛋白聚糖 I 类（biglycan、decorin、asporin、ECM2 和 ECMX）和 II 类（fibromodulin、lumican、prolargin、keratocan 和 osteoadherin）的分布。这些化合物的分布如下：内芯表达 decorin、biglycan、lumican、fibromodulin 和 osteoadherin；中间层显示 osteoadherin 免疫反应性；外芯 biglycan、decorin、lumican、fibromodulin 和 osteoadherin；囊含有 biglycan、decorin、fibromodulin 和 lumican。asporin、prolargin 和 keratocan 无法检测到。这些结果补充了我们对人类帕西尼小体中小富含亮氨酸的蛋白聚糖分布的认识，并有助于了解这些感觉形成物中细胞外基质的组成。
