College of Food & Biology Engineering, Zhejiang Gongshang University, Hangzhou, Zhejiang 310035, China; Zhejiang Provincial Key Lab for Chem & Bio Processing Technology of Agricultural Products, Zhejiang University of Science and Technology, Hangzhou, Zhejiang, 310023, China.
College of Food & Biology Engineering, Zhejiang Gongshang University, Hangzhou, Zhejiang 310035, China.
Colloids Surf B Biointerfaces. 2019 Jul 1;179:488-494. doi: 10.1016/j.colsurfb.2019.03.055. Epub 2019 Mar 27.
Vesicles possess unique biofilm structures and offer biomimetic advantages for drug and gene delivery. Herein, we report the spontaneous vesicle formation from ultrashort alkyl-phosphonic acids in the presence of amino acids. The aggregation characteristics and self-assembly structures of vesicles in aqueous solution were investigated by using dynamic light scattering, zeta potential, and cryo-transmission electron microscopy. We combined low-field nuclear magnetic resonance and Fourier transform infrared spectroscopy to study the H-proton-induced multilamellar vesicle formation. When we increased the molar fraction of serine, stable and closed spherical vesicles were formed at relatively low critical micelle concentrations. This transition of the self-assembled structure indicates that vesicle formation occurs when the chain length and the magnitude of the surface charge cause a fluctuation in the volume of the vesicle. Density functional theory reveals the critical role of the mixed alkyl-phosphonic acid/amino acid-enhanced electrostatic attraction between the head groups and hydrogen bonds associated with the aggregated states.
囊泡具有独特的生物膜结构，并为药物和基因传递提供仿生优势。本文报道了在氨基酸存在的情况下，超短链烷基膦酸自发形成囊泡。通过动态光散射、动电电位和冷冻透射电子显微镜研究了囊泡在水溶液中的聚集特性和自组装结构。我们结合低场核磁共振和傅里叶变换红外光谱研究了 H-质子诱导的多层囊泡形成。当我们增加丝氨酸的摩尔分数时，在相对较低的临界胶束浓度下形成稳定的闭球形囊泡。这种自组装结构的转变表明，当链长和表面电荷的大小导致囊泡体积波动时，囊泡形成。密度泛函理论揭示了混合烷基膦酸/氨基酸增强头基之间的静电吸引力以及与聚集态相关的氢键的关键作用。
