一种用于白细胞介素-2活性的简易微量测定法。
A simple microassay for interleukin-2 activity.
作者信息
Ando I, Beverley P C
出版信息
J Immunol Methods. 1987 Jan 26;96(1):133-7. doi: 10.1016/0022-1759(87)90377-2.
PMID:3100647
Abstract
A simple in vitro assay is described to quantitate human interleukin-2 (IL-2). It is based on the spontaneous' activation of human peripheral T cells in tissue culture and the subsequent expression of the IL-2 receptor. The results obtained with this assay agree with those of assays which utilise IL-2 dependent T cell clones. The method is highly sensitive, reproducible and easy to perform.
摘要
描述了一种用于定量人白细胞介素-2（IL-2）的简单体外测定法。它基于组织培养中人外周血T细胞的自发激活以及随后IL-2受体的表达。该测定法获得的结果与使用IL-2依赖性T细胞克隆的测定法结果一致。该方法高度灵敏、可重复且易于操作。
Zotero 插件