Havell E A
J Interferon Res. 1986 Oct;6(5):489-97. doi: 10.1089/jir.1986.6.489.
The R4-6A2 rat-mouse hybridoma, which secretes a rat IgG1 monoclonal antibody (MAb) capable of neutralizing murine interferon-gamma (MuIFN-gamma), was grown as ascites in athymic nude (nu/nu) mice. The neutralizing titers of ascitic fluids were 400 times higher than those of supernatants from R4-6A2 cultures. Moreover, the specific activities (MAb) neutralizing units/mg protein) of the ascitic fluids were much greater than those of R4-6A2 culture supernatants. Ascitic fluid MAb was purified by anion-exchange chromatography, with excellent recovery of applied neutralizing activity. The ability of the R4-6A2 anti-MuIFN-gamma MAb to neutralize several different activities of MuIFN-gamma was studied. The MAb neutralized the ability of MuIFN-gamma to inhibit the growth of cells (antiproliferative activity). The ability of MuIFN-gamma to render cells of a heterologous species (rat) resistant to viral replication was also neutralized by this MAb. The MAb-neutralizing titers for MuIFN-gamma antiviral activities, on both homologous (murine L929) and heterologous (rat fibroblast) cells, were inversely proportional to the antiviral activity titers on each cell type.
R4-6A2大鼠-小鼠杂交瘤细胞分泌一种能够中和小鼠干扰素-γ（MuIFN-γ）的大鼠IgG1单克隆抗体（MAb），该杂交瘤细胞在无胸腺裸鼠（nu/nu）体内以腹水形式生长。腹水的中和效价比R4-6A2培养上清液高400倍。此外，腹水的比活性（MAb中和单位/毫克蛋白）远高于R4-6A2培养上清液。通过阴离子交换色谱法纯化腹水MAb，所施加的中和活性回收率良好。研究了R4-6A2抗MuIFN-γ MAb中和MuIFN-γ几种不同活性的能力。该MAb中和了MuIFN-γ抑制细胞生长的能力（抗增殖活性）。该MAb还中和了MuIFN-γ使异源物种（大鼠）细胞对病毒复制产生抗性的能力。在同源（小鼠L929）和异源（大鼠成纤维细胞）细胞上，该MAb对MuIFN-γ抗病毒活性的中和效价与每种细胞类型上的抗病毒活性效价呈反比。
