Department of Sports Medicine and Joint Surgery, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Hokkaido, Japan; Sports and Arthroscopy Center, Hanna Central Hospital, Ikoma, Nara, Japan.
Department of Advanced Therapeutic Research for Sports Medicine, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Hokkaido, Japan; Department of Orthopaedic Surgery, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Hokkaido, Japan.
Orthop Traumatol Surg Res. 2019 Jun;105(4):683-690. doi: 10.1016/j.otsr.2018.12.017. Epub 2019 Apr 18.
Implantation of autogenous meniscal fragments wrapped with a fascia sheath significantly enhances fibrocartilage regeneration in vivo in defect cases at 12 weeks after implantation. The specific effects of the implanted autologous living chondrocytes and meniscal matrix have not been elucidated, however. The aim of this study was to clarify the role of autologous living chondrocytes contained in the meniscal matrix in in vivo meniscus regeneration induced by in situ meniscus fragment implantation.
Implantation of meniscus fragments containing autologous living chondrocytes may result in significant in vivo meniscus regeneration.
Seventy-five rabbits were used in this study. A partial meniscectomy of the anterior one-third of the medial meniscus including the part of the anterior horn was performed. The rabbits were divided into 3 groups. In Group I, no treatment was applied to the defect. In Group II, the autogenous meniscal fragments devitalized by freeze-thaw treatment were reimplanted into the defect. In Group III, the autogenous meniscal fragments were reimplanted. In each group, the defect was covered with a fascia. Five rabbits from each group were subjected to morphologic and histologic evaluations at 3, 6, and 12 weeks, and 5 rabbits from each group were subjected to biomechanical evaluations at 6 and 12 weeks.
Histologically, no cells were seen in the grafted meniscal fragments at 3 weeks in Group II, whereas chondrocytes in the grafted meniscal fragments were alive at 3 weeks in Group III. Histologic and biomechanical data for Group II were slightly but significantly better than those of Group I at 12 weeks after implantation (p=0.007 and p=0.002, respectively), whereas the data for Group III were significantly superior to those of Groups I and II at 12 weeks (p<0.0014 and p<0.0029, respectively).
Grafted autologous living chondrocytes contained in the meniscal matrix play an important role in in vivo meniscus regeneration induced by in situ meniscus fragment implantation.
II, Controlled laboratory study.
将自体半月板碎片包裹在筋膜鞘中进行植入，可显著促进植入后 12 周时体内半月板缺损处纤维软骨的再生。然而，植入的自体活软骨细胞和半月板基质的具体作用尚未阐明。本研究旨在阐明半月板基质中包含的自体活软骨细胞在原位半月板碎片植入诱导的体内半月板再生中的作用。
植入包含自体活软骨细胞的半月板碎片可能导致显著的体内半月板再生。
本研究共使用了 75 只兔子。对内侧半月板前 1/3 包括前角的部分进行部分半月板切除术。兔子被分为 3 组。在第 I 组中，不进行任何治疗。在第 II 组中，将经过冻融处理失活的自体半月板碎片重新植入缺损处。在第 III 组中，将自体半月板碎片重新植入。每组缺损均用筋膜覆盖。每组中有 5 只兔子分别在 3、6 和 12 周时进行形态学和组织学评估，每组中有 5 只兔子分别在 6 和 12 周时进行生物力学评估。
组织学上，第 II 组在 3 周时未见移植物半月板碎片中有细胞，而第 III 组在 3 周时移植物半月板碎片中的软骨细胞仍然存活。植入后 12 周时，第 II 组的组织学和生物力学数据略优于第 I 组（p=0.007 和 p=0.002），而第 III 组的数据明显优于第 I 组和第 II 组（p<0.0014 和 p<0.0029）。
原位半月板碎片植入诱导的体内半月板再生中，半月板基质中包含的植入自体活软骨细胞发挥了重要作用。
II 期，对照实验室研究。
Zotero 插件