兔模型中自体半月板碎片再生半月板无血管区。
Regeneration of meniscal avascular zone using autogenous meniscal fragments in a rabbit model.
机构信息
Department of Orthopaedics, Loudi Central Hospital, No.51 Changqing Central Street, Lou Xing District, Loudi, 417000, Hunan, China.
Department of Orthopaedics, First Affiliated Hospital of South China University, NO.69 Chuangshan Road, Hengyang, 421000, Hunan, China.
出版信息
BMC Surg. 2022 May 28;22(1):209. doi: 10.1186/s12893-022-01663-3.
BACKGROUND
To investigate the effects of autologous meniscus fragment (AMF) implantation on injury in the meniscal avascular zone in mature rabbits.
METHODS
Adult New Zealand white rabbits were randomly divided into two groups. Massive one-piece meniscus tissue was implanted in situ as control. In the experimental group, AMF was used to repair the meniscal injury in the avascular zone. Meniscal damage was assessed by gross observation of the degree of healing and histological semi-quantitative evaluation within 12 weeks postoperatively. The healing of meniscus interface was assessed by gross observation semiquantitative scoring and microscopic examination hematoxylin and eosin (H&E) staining at 2, 4, 8, and 12 weeks after surgery. The expressions of proliferating cell nuclear antigen (PCNA), collagen type I (COL1A1), and collagen type II (COL2) were detected by immunohistochemical staining.
RESULTS
The degree of healing in the AMF group showed a significant increase over time (P < 0.05); the AMF group showed higher gross scores than the control group at 4, 8, and 12 weeks after surgery (P < 0.05). The histological scores in the AMF group were significantly higher than those in the control group at 4, 8, and 12 weeks after surgery (P < 0.05). The protein expression of PCNA in the AMF group was greater than that in the control group at 2, 4, and 8 weeks after surgery (P < 0.05). In addition, compared with the control group, the protein levels of COL1A1 and COL2 were significantly upregulated at each time-point. At 2 and 4 weeks after surgery, the expression level of COL1A1 increased in both groups followed by a gradual decrease after 8 weeks (P < 0.05). At 2, 4, 8, and 12 weeks after surgery, the expression levels of COL2 showed a gradual decrease in both groups (P < 0.05).
CONCLUSIONS
Our study demonstrated that the AMF method can promote the repair of rabbit meniscal injury in the avascular zone, and this method may potentially be used for clinical application.
背景
为了研究自体半月板碎片（AMF）植入对成熟兔半月板无血管区损伤的影响。
方法
成年新西兰白兔随机分为两组。对照组大块半月板组织原位植入。实验组采用 AMF 修复半月板无血管区损伤。术后 12 周内通过大体观察愈合程度和组织学半定量评价评估半月板损伤。术后 2、4、8 和 12 周，通过大体观察半定量评分和苏木精-伊红（H&E）染色显微镜检查评估半月板界面愈合情况。通过免疫组织化学染色检测增殖细胞核抗原（PCNA）、Ⅰ型胶原（COL1A1）和Ⅱ型胶原（COL2）的表达。
结果
AMF 组的愈合程度随时间显著增加（P < 0.05）；术后 4、8 和 12 周，AMF 组大体评分均高于对照组（P < 0.05）。术后 4、8 和 12 周，AMF 组组织学评分均显著高于对照组（P < 0.05）。术后 2、4 和 8 周，AMF 组 PCNA 蛋白表达均高于对照组（P < 0.05）。此外，与对照组相比，各时间点 COL1A1 和 COL2 蛋白水平均显著上调。术后 2 和 4 周，两组 COL1A1 表达均升高，8 周后逐渐下降（P < 0.05）。术后 2、4、8 和 12 周，两组 COL2 表达水平逐渐下降（P < 0.05）。
结论
本研究表明，AMF 方法可促进兔半月板无血管区损伤的修复，该方法可能具有潜在的临床应用价值。
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