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用于热敏生物分子的膜冷冻浓缩混合技术。研究、应用及技术经济效益。

Membrane-Freeze Concentration Hybrid for Temperature-Sensitive Biomolecules. Investigation, Application, and Techno-Economic Benefits.

作者信息

van Beek Timo, Budde Michael, van Esch Jan

机构信息

GEA Technology Center for Freeze Concentration and Melt Crystallization de Beverspijken 7B 5221 's Hertogenbosch The Netherlands.

BASF SE G-ENB/OP - O819, Phytase factory Carl-Bosch-Strasse 38 67056 Ludwigshafen, Germany.

出版信息

Chem Eng Technol. 2018 Dec;41(12):2385-2392. doi: 10.1002/ceat.201800318. Epub 2018 Oct 31.

DOI:10.1002/ceat.201800318
PMID:31007405
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6472580/
Abstract

In order to close the technology gap between membrane technologies and spray/freeze-drying ideally with a technology that avoids thermal stress to sensitive enzyme solutions, the limits of freeze concentration for this application have been investigated. On laboratory scale it was found that average crystal sizes are > 300 µm despite high viscosity and ice separation is possible up to 42 % solids and > 1000 mms viscosity. No activity loss was observed during concentration. A combination of two-stage freeze concentration with a filter and wash column for ice liquid separation in an integrated setup with ultrafiltration has the greatest potential and was shown to be economically feasible in three out of four cases studied.

摘要

为了用一种避免对敏感酶溶液产生热应力的技术，理想地弥合膜技术与喷雾/冷冻干燥之间的技术差距，已对该应用中冷冻浓缩的极限进行了研究。在实验室规模上发现，尽管粘度很高，但平均晶体尺寸大于300 µm，并且可以实现高达42%固体含量的冰分离，粘度大于1000 mms。浓缩过程中未观察到活性损失。在与超滤集成的装置中，将两级冷冻浓缩与用于冰液分离的过滤器和洗涤柱相结合具有最大潜力，并且在所研究的四个案例中有三个案例显示在经济上是可行的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38f6/6472580/27e750319554/CEAT-41-2385-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38f6/6472580/5b61daf1fdf2/CEAT-41-2385-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38f6/6472580/7e035153b7ef/CEAT-41-2385-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38f6/6472580/0ca74e37133e/CEAT-41-2385-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38f6/6472580/d43f5132e670/CEAT-41-2385-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38f6/6472580/64d11498cee8/CEAT-41-2385-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38f6/6472580/468a69a6e18e/CEAT-41-2385-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38f6/6472580/43c90d16e2f7/CEAT-41-2385-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38f6/6472580/27e750319554/CEAT-41-2385-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38f6/6472580/5b61daf1fdf2/CEAT-41-2385-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38f6/6472580/7e035153b7ef/CEAT-41-2385-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38f6/6472580/0ca74e37133e/CEAT-41-2385-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38f6/6472580/d43f5132e670/CEAT-41-2385-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38f6/6472580/64d11498cee8/CEAT-41-2385-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38f6/6472580/468a69a6e18e/CEAT-41-2385-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38f6/6472580/43c90d16e2f7/CEAT-41-2385-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38f6/6472580/27e750319554/CEAT-41-2385-g008.jpg

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The roots--a short history of industrial microbiology and biotechnology.根——工业微生物学和生物技术简史。
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