Chaubey Lavina, Kumar Deepak, Prakash Vidyut, Nath Gopal
Department of Obstetrics and Gynaecology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
J Hum Reprod Sci. 2019 Jan-Mar;12(1):35-39. doi: 10.4103/jhrs.JHRS_149_17.
The aim of this study is to compare the results of nested polymerase chain reaction (PCR) for early detection of genital tuberculosis (GTB) using menstrual blood (MB) and endometrial tissue (ET) as samples in females presenting as infertility.
The ET and MB samples were collected from a total of 194 females, enrolled in this study. DNA isolation from samples was done using standard, phenol-chloroform method. Heat shock protein gene (/) of was targeted and amplified, and the final products were analyzed.
Overall, 126 (65%) cases of infertility were positive for complex by nested PCR. The detection rates in the two samples were statistically insignificant. The combined positivity rate of ET and MB, when compared with positivity rate in MB showed a positive predictive value, negative predictive value, and accuracy of 100%, 68.7%, and 84%, respectively. The results of nested PCR using MB as sample alone showed good agreement with the nested PCR results of the combined samples.
The Nested PCR of MB can be used as a noninvasive screening test for early diagnosis of GTB.
本研究旨在比较以月经血（MB）和子宫内膜组织（ET）为样本，采用巢式聚合酶链反应（PCR）对表现为不孕的女性进行生殖器结核（GTB）早期检测的结果。
本研究共纳入194名女性，采集其ET和MB样本。采用标准的酚 - 氯仿法从样本中提取DNA。靶向扩增结核分枝杆菌的热休克蛋白基因（/），并对最终产物进行分析。
总体而言，126例（65%）不孕病例通过巢式PCR检测结核分枝杆菌复合物呈阳性。两个样本中的检测率在统计学上无显著差异。与MB中的阳性率相比，ET和MB的联合阳性率显示其阳性预测值、阴性预测值和准确率分别为100%、68.7%和84%。单独以MB为样本的巢式PCR结果与联合样本的巢式PCR结果显示出良好的一致性。
MB的结核分枝杆菌巢式PCR可作为GTB早期诊断的非侵入性筛查试验。
