Bahey Noha Gamal, Abd Elaziz Hekmat Osman, Elsayed Gadalla Kamal Kamal
Department of Histology and Cell Biology, Faculty of Medicine, Tanta University, Egypt.
Department of Histology, Faculty of Medicine, Sohag University, Egypt.
J Microsc Ultrastruct. 2019 Jan-Mar;7(1):1-8. doi: 10.4103/JMAU.JMAU_53_18.
Bisphenol A (BPA) is intensely used in the production of polycarbonate plastics and epoxy resins. Recently, BPA has been receiving increased attention due to its link to various health problems that develop after direct or indirect human exposure. Previous studies have shown the harmful effect of high doses of BPA; however, the effect of small doses of BPA on disease development is controversial. The aim of this study was to investigate the effect of a low dose of BPA on the rat myocardium and to explore the outcome of coadministration of Omega-3 fatty acid (FA). Thirty adult male rats were divided equally into control group, BPA-treated group (1.2 mg/kg/day, intraperitoneally for 3 weeks), and BPA and Omega-3-treated group (received BPA as before plus Omega-3 at a daily dose of 300 mg/kg/day orally) for 3 weeks. Exposure to BPA resulted in structural anomalies in the rat myocardium in the form of disarrangement of myofibers, hypertrophy of myocytes, myocardial fibrosis, and dilatation of intramyocardial arterioles. On the other hand, mast cell density and media-to-lumen area ratio were not significantly altered. Interestingly, concomitant administration of Omega-3 FAs with BPA significantly reduced BPA-induced changes and provided a protective effect to the myocardium. In conclusion, exposure to a low dose of BPA could potentially lead to pathological alterations in the myocardium, which could be prevented by administration of Omega-3 FA.
双酚A（BPA）被广泛应用于聚碳酸酯塑料和环氧树脂的生产中。近年来，由于其与人类直接或间接接触后出现的各种健康问题有关，BPA受到了越来越多的关注。先前的研究已经表明高剂量BPA的有害影响；然而，低剂量BPA对疾病发展的影响仍存在争议。本研究的目的是调查低剂量BPA对大鼠心肌的影响，并探索联合给予ω-3脂肪酸（FA）的结果。将30只成年雄性大鼠平均分为对照组、BPA处理组（1.2mg/kg/天，腹腔注射3周）和BPA与ω-3处理组（按之前方式给予BPA，另加每日口服300mg/kg/天的ω-3），持续3周。暴露于BPA导致大鼠心肌出现结构异常，表现为肌纤维排列紊乱、心肌细胞肥大、心肌纤维化以及心肌内小动脉扩张。另一方面，肥大细胞密度和中膜与管腔面积比没有显著改变。有趣的是，ω-3脂肪酸与BPA联合给药显著减少了BPA诱导的变化，并对心肌起到了保护作用。总之，暴露于低剂量BPA可能会导致心肌发生病理改变，而给予ω-3脂肪酸可以预防这种改变。
