Moodley Preshanthan, Gueguim Kana E B
University of KwaZulu-Natal, School of Life Sciences, Pietermaritzburg, South Africa.
Biotechnol Rep (Amst). 2019 Mar 26;22:e00329. doi: 10.1016/j.btre.2019.e00329. eCollection 2019 Jun.
This study examines the kinetics of BY4743 growth and bioethanol production from sugarcane leaf waste (SLW), utilizing two different optimized pretreatment regimes; under two fermentation modes: steam salt-alkali filtered enzymatic hydrolysate (SSA-F), steam salt-alkali unfiltered (SSA-U), microwave salt-alkali filtered (MSA-F) and microwave salt-alkali unfiltered (MSA-U). The kinetic coefficients were determined by fitting the Monod, modified Gompertz and logistic models to the experimental data with high coefficients of determination R > 0.97. A maximum specific growth rate (μ ) of 0.153 h was obtained under SSA-F and SSA-U whereas, 0.150 h was observed with MSA-F and MSA-U. SSA-U gave a potential maximum bioethanol concentration (P) of 31.06 g/L compared to 30.49, 23.26 and 21.79 g/L for SSA-F, MSA-F and MSA-U respectively. An insignificant difference was observed in the μ and P for the filtered and unfiltered enzymatic hydrolysate for both SSA and MSA pretreatments, thus potentially reducing a unit operation. These findings provide significant insights for process scale up.
本研究考察了BY4743利用甘蔗叶废料（SLW）生长及生产生物乙醇的动力学，采用了两种不同的优化预处理方案；在两种发酵模式下：蒸汽盐碱过滤酶解液（SSA-F）、蒸汽盐碱未过滤（SSA-U）、微波盐碱过滤（MSA-F）和微波盐碱未过滤（MSA-U）。通过将莫诺德模型、修正的冈珀茨模型和逻辑模型与测定系数R>0.97的实验数据进行拟合来确定动力学系数。在SSA-F和SSA-U条件下获得的最大比生长速率（μ）为0.153 h，而在MSA-F和MSA-U条件下观察到的为0.150 h。SSA-U的潜在最大生物乙醇浓度（P）为31.06 g/L，而SSA-F、MSA-F和MSA-U的分别为30.49、23.26和21.79 g/L。对于SSA和MSA预处理，过滤和未过滤的酶解液在μ和P方面观察到的差异不显著，因此有可能减少一个单元操作。这些发现为工艺放大提供了重要见解。
