Cherepakhin V V, Iakubov L Z, Ibragimov A R, Vengerova T I, Rokhlin O V
Mol Biol (Mosk). 1986 Nov-Dec;20(6):1672-81.
The investigation of 750 B-lymphocyte hybridoma clones obtained by fusion of mouse myeloma and newborn heterozygous Igk-la/Igk-1b rat splenocytes has revealed that 9,8% of Ig kappa-chain genes are rearranged productively. Seventeen hybridomas secrete kappa-chains of both allelic variants. The analysis of IgM molecules of nine such clones demonstrated that in six cases only one L-chain allotype is present in IgM. Thus for the first time the high frequency of selective association of H and L chains was shown. Evidently this selectively may function as one of the allelic exclusion mechanisms at the Ig assembly stage.
对通过小鼠骨髓瘤与新生杂合 Igk-la/Igk-1b 大鼠脾细胞融合获得的 750 个 B 淋巴细胞杂交瘤克隆进行的研究表明，9.8%的 Ig κ链基因发生了有效重排。17 个杂交瘤分泌两种等位基因变体的 κ链。对九个此类克隆的 IgM 分子分析表明，在六个案例中，IgM 中仅存在一种 L 链同种异型。因此，首次显示了 H 链和 L 链选择性关联的高频率。显然，这种选择性可能在 Ig 组装阶段作为等位基因排斥机制之一发挥作用。
