Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China.
Int J Mol Sci. 2019 Apr 21;20(8):1954. doi: 10.3390/ijms20081954.
Purple acid phosphatase (PAP) encoding genes are a multigene family. PAPs require iron (Fe) to exert their functions that are involved in diverse biological roles including Fe homeostasis. However, the possible roles of PAPs in response to excess Fe remain unknown. In this study, we attempted to understand the regulation of PAPs by excess Fe in tea plant (). A genome-wide investigation of PAP encoding genes identified 19 members based on the conserved motifs. The phylogenetic analysis showed that PAPs could be clustered into four groups, of which group II contained two specific cysteine-containing motifs "GGECGV" and "YERTC". To explore the expression patterns of genes in response to excessive Fe supply, RNA-sequencing (RNA-seq) analyses were performed to compare their transcript abundances between tea plants that are grown under normal and high iron conditions, respectively. 17 members were shown to be transcribed in both roots and leaves. When supplied with a high amount of iron, the expression levels of four genes were significantly changed. Of which, , and were shown as downregulated, while the highly expressed was upregulated. Moreover, was found to be alternatively spliced, suggesting its post-transcriptional regulation. The present work implicates that some genes could be associated with the responses of tea plants to the iron regime, which may offer a new direction towards a further understanding of iron homeostasis and provide the potential approaches for crop improvement in terms of iron biofortification.
紫色酸性磷酸酶（PAP）编码基因是一个多基因家族。PAP 需要铁（Fe）来发挥其功能，这些功能涉及多种生物学作用，包括铁稳态。然而，PAP 在应对过量 Fe 时的可能作用仍然未知。在这项研究中，我们试图了解茶树（）中过量 Fe 对 PAP 编码基因的调控。基于保守基序，对 PAP 编码基因进行了全基因组研究，鉴定出了 19 个成员。系统发育分析表明，PAP 可以聚类为四个组，其中组 II 包含两个特定的含半胱氨酸基序“GGECGV”和“YERTC”。为了探索基因在过量 Fe 供应下的表达模式，进行了 RNA-seq 分析，以比较它们在正常和高铁条件下生长的茶树之间的转录丰度。结果显示，17 个成员在根和叶中均有转录。当供应大量铁时，四个基因的表达水平发生显著变化。其中，、和被下调，而高度表达的则被上调。此外，还发现了的可变剪接，提示其转录后调控。本研究表明，一些基因可能与茶树对铁调控的反应有关，这可能为进一步理解铁稳态提供新的方向，并为作物改良提供铁生物强化的潜在途径。
