Liu Hong-Chao, Rao Lei, Meng Jia-Hui, Zuo Wen-Teng, Sun Ting-Ting
College of Horticulture, China Agricultural University, Beijing 100193, China.
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
Int J Mol Sci. 2025 Jan 24;26(3):1011. doi: 10.3390/ijms26031011.
Purple acid phosphatases (PAPs) play a significant role in plant phosphorus nutrition and can not only release phosphorus from the soil but also regulate the distribution of phosphorus in plants throughout their entire growth and development process. Moreover, members of the PAP protein family exert a more extensive influence on plant mineral homeostasis, developmental processes, and stress responses. Three clusters of purple acid phosphatases, including 31 putative genes, were identified in apples () by searching the Genome Database for Rosaceae. The structure, chromosomal distribution and location, phylogeny, motifs, and cis-acting elements in the gene promoter regions of the gene family are reviewed. These genes exhibit different expression patterns in different tissues. For example, almost all genes are strongly expressed in the roots, except for , , and . Similarily, all were expressed in the leaves while the transcript levels of , , , , , , , and were highest in apple flowers. Overall, the expression of the 31 genes significantly changed in either the roots or leaves following the application of phosphorus and/or drought stress. These results indicate that MdPAP family members play a role in plant adaptation to adverse environments. This work explores the adaptative responses to phosphorus and/or drought conditions in apple and establishes a foundation for an enhanced comprehension of the evolution of PAP families and the exploration of the genes of interest.
紫色酸性磷酸酶（PAPs）在植物磷营养中发挥着重要作用，不仅能从土壤中释放磷，还能在植物整个生长发育过程中调节磷在植物体内的分布。此外，PAP蛋白家族成员对植物矿物质稳态、发育过程和胁迫反应有着更广泛的影响。通过搜索蔷薇科基因组数据库，在苹果（）中鉴定出了三簇紫色酸性磷酸酶，包括31个推定基因。本文综述了该基因家族基因启动子区域的结构、染色体分布与定位、系统发育、基序和顺式作用元件。这些基因在不同组织中表现出不同的表达模式。例如，几乎所有基因在根中强烈表达，但除外。同样，所有基因在叶中表达，而、、、、、、和在苹果花中的转录水平最高。总体而言，在施加磷和/或干旱胁迫后，31个基因在根或叶中的表达均发生了显著变化。这些结果表明，MdPAP家族成员在植物适应逆境环境中发挥作用。本研究探索了苹果对磷和/或干旱条件的适应性反应，为深入理解PAP家族的进化和挖掘相关基因奠定了基础。
