Key Laboratory of Aquatic Genomics, Ministry of Agriculture, Beijing, China.
Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao, China.
Mol Reprod Dev. 2019 Jun;86(6):727-737. doi: 10.1002/mrd.23150. Epub 2019 Apr 22.
Double haploidy is an advantageous situation for genetic mapping and genome sequencing studies. In the present study, the hypothalamus and pituitary gland from sterile and fertile double-haploid (DH) Japanese flounders (aged 5 years) were used as experimental materials for studying the expression of genes in individuals with reproductive disorders, using high-throughput sequencing technology. The results revealed abnormal levels of some hormones in sterile DHs during the breeding season. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the significantly different microRNAs and messenger RNAs were related to metabolism, signal transduction, and melanogenesis; those related to steroid hormone synthesis and secretion related pathways were not detected. Our results suggest that the key to sterility in DHs was the arrested ovary development. However, the reason for arrested ovary development was mainly related to the lower levels of expression of genes involved in steroid biosynthesis in gonads, and was not related to the pituitary. For maintaining homeostasis, the hypothalamus and pituitary would have large differences in several processes, including signal transduction, metabolism, and immune response. The present study provides primary data for further studies on sterility in fish, and even in other animals.
双单倍体是遗传图谱和基因组测序研究的有利条件。在本研究中，使用来自不育和可育双单倍体（DH）日本牙鲆（5 岁）的下丘脑和垂体作为实验材料，使用高通量测序技术研究生殖障碍个体的基因表达。结果表明，在繁殖季节不育 DH 中的一些激素水平异常。基因本体论和京都基因与基因组百科全书通路分析表明，显著差异的 microRNAs 和信使 RNAs 与代谢、信号转导和黑色素生成有关；未检测到与类固醇激素合成和分泌相关途径相关的差异基因。我们的研究结果表明，DH 不育的关键是卵巢发育停滞。然而，卵巢发育停滞的原因主要与性腺中类固醇生物合成相关基因表达水平较低有关，而与垂体无关。为了维持体内平衡，下丘脑和垂体在信号转导、代谢和免疫反应等几个过程中会有很大的差异。本研究为鱼类甚至其他动物的不育研究提供了初步数据。
