Umehara K, Kuno-Sakai H, Iwasaki H, Takesue R, Hoshi N, Furukawa T, Ikeda K, Yabe Y, Kimura M, Leung W C
Tokai J Exp Clin Med. 1986 Mar;11(1):19-21.
We tried to isolate pathogenic viruses from specimens of patients with Kawasaki disease. Blood clots and sera, spinal fluid, throat swabs, stool and urine from 24 patients with Kawasaki disease were studied. The specimens were inoculated into HEL cells and Vero cells. The cells were observed for one month, but no cytopathic effect (CPE) occurred. Blind passage was performed, but no degeneration of the second series of cells was observed. Fluorescent antibody indirect methods to determine viral antigens in cells inoculated with patients' specimens was also negative.
我们试图从川崎病患者的标本中分离出致病病毒。对24例川崎病患者的血凝块和血清、脑脊液、咽拭子、粪便及尿液进行了研究。将标本接种到HEL细胞和Vero细胞中。对细胞观察了一个月，但未出现细胞病变效应（CPE）。进行了盲传，但未观察到第二代细胞发生变性。采用荧光抗体间接法检测接种患者标本的细胞中的病毒抗原，结果也为阴性。
