a Department of Immunology School of Elementary Medicine, North China University of Science and Technology , Tangshan , China.
b North China University of Science and Technology, Hebei Key Laboratory for Chronic Diseases , Tangshan , China.
Int J Radiat Biol. 2019 Aug;95(8):1178-1184. doi: 10.1080/09553002.2019.1607605. Epub 2019 May 9.
The present study aims to evaluate the effect of cell phone radiation on neutrophil of mice. 40 male BALB/C mice were randomly divided into four groups as control, blank control, TD-CDMA, and LTE-advanced groups, respectively. Mice were exposed to cell phone radiation for a period of 6 weeks. Then numbers of neutrophil were detected by fully automatic hematology analyzer. Soft agar diffusion method was performed to assess the chemotaxis of neutrophils while the phagocytosis of neutrophils was determined by measuring the phagocytosis percentage. Apoptosis was analyzed by flow cytometry. No significant differences were observed among the control and exposure groups regarding the numbers of neutrophils after 2 weeks' exposure to cell phone radiation, while the numbers of neutrophils in TD-SCDMA and LTE-advanced groups were seen to rise after an exposure of 4 or 6 weeks. No effect was observed on chemotaxis of neutrophils due to phone radiation. The phagocytosis of neutrophils was decreased while the apoptosis were increased both in TD-SCDMA and LTE-advanced groups after 6 weeks exposure. Mobile phone radiation could give rise to increase of neutrophil numbers yet with no effect whatever on neutrophils chemotaxis, and the radiation was likely to cause decrease of phagocytosis and induced apoptosis of neutrophils.
本研究旨在评估手机辐射对小鼠中性粒细胞的影响。将 40 只雄性 BALB/C 小鼠随机分为对照组、空白对照组、TD-CDMA 组和 LTE-advanced 组,分别进行研究。将小鼠暴露于手机辐射下 6 周。然后使用全自动血液分析仪检测中性粒细胞数量。使用软琼脂扩散法评估中性粒细胞的趋化性,通过测量吞噬百分率来确定中性粒细胞的吞噬作用。通过流式细胞术分析细胞凋亡。在暴露于手机辐射 2 周后,对照组和暴露组之间的中性粒细胞数量没有明显差异,而在 TD-SCDMA 和 LTE-advanced 组中,暴露 4 或 6 周后中性粒细胞数量上升。手机辐射对中性粒细胞的趋化性没有影响。暴露 6 周后,TD-SCDMA 和 LTE-advanced 组的中性粒细胞吞噬作用下降,凋亡增加。手机辐射可引起中性粒细胞数量增加,但对中性粒细胞趋化性无影响,辐射可能导致吞噬作用下降,并诱导中性粒细胞凋亡。