马来西亚牛、羊和山羊泰勒虫病的首次分子调查。
The first molecular survey of theileriosis in Malaysian cattle, sheep and goats.
作者信息
Kho Kai Ling, Amarajothi Asha Devi G, Koh Fui Xian, Panchadcharam Chandrawathani, Hassan Nizam Quaza Nizamuddin, Tay Sun Tee
机构信息
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Veterinary Research Institute, 59, Jalan Sultan Azlan Shah, 31400 Ipoh, Perak, Malaysia.
出版信息
Vet Parasitol Reg Stud Reports. 2017 Dec;10:149-153. doi: 10.1016/j.vprsr.2017.08.003. Epub 2017 Aug 8.
This study reports the molecular detection of Theileria spp. from six cattle farms, a sheep farm and a goat farm located at different states in Peninsular Malaysia. Animal blood samples were screened for the presence of Theileria DNA using a conventional polymerase chain reaction (PCR) assay. A total of 155 (69.2%) of 224 cattle investigated were PCR-positive for Theileria DNA. The occurrences of Theileria spp. ranged from 17.5% to 100.0% across six cattle farms. Theileria DNA was detected from 90.0% of 40 sheep but none of 40 goats examined in this study. Sequence analyses of amplified 18S rRNA partial fragments (335-338bp) confirmed the identification of Theileria buffeli, Theileria sergenti, and Theileria sinensis in representative samples of cattle and ticks. T. luwenshuni was identified in the infected sheep. The high occurrences of Theileria spp. in our farm animals highlight the needs for appropriate control and preventive measures for theileriosis.
本研究报告了在马来西亚半岛不同州的六个养牛场、一个养羊场和一个养山羊场中对泰勒虫属的分子检测情况。使用常规聚合酶链反应（PCR）检测方法对动物血液样本进行泰勒虫DNA检测。在调查的224头牛中，共有155头（69.2%）泰勒虫DNA检测呈阳性。六个养牛场中泰勒虫属的出现率在17.5%至100.0%之间。在本研究中，从40只绵羊中的90.0%检测到了泰勒虫DNA，但在40只山羊中均未检测到。对扩增的18S rRNA部分片段（335 - 338bp）进行序列分析，证实了在牛和蜱的代表性样本中鉴定出了水牛泰勒虫、瑟氏泰勒虫和中华泰勒虫。在感染的绵羊中鉴定出了吕氏泰勒虫。我们农场动物中泰勒虫属的高出现率凸显了对泰勒虫病采取适当控制和预防措施的必要性。
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