Badri Milad, Eslahi Aida Vafae, Majidiani Hamidreza, Pirestani Majid
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Vet Parasitol Reg Stud Reports. 2017 Dec;10:58-61. doi: 10.1016/j.vprsr.2017.08.004. Epub 2017 Aug 8.
The zoonotic helminth, Spirometra, has several species with almost global distribution. Herein, we describe the first detailed molecular detection of Spirometra erinaceieuropaei in a road-killed wildcat (Felis silvestris) in Iran and its identification at the species level using CO1 gene. Genomic DNA was extracted using CTAB extraction method. The DNA then was applied for PCR amplification of cytochrome c oxidase subunit I (CO1) gene. Afterwards, PCR product was sequenced and obtained data were analyzed and multiple aligned using BLAST program, ClustalX and Bioedit software. Microscopy findings and diagnostic clues revealed that the parasite is a Spirometra sp. cestode. Consequently, molecular analysis on the basis of cytochrome c oxidase subunit 1 (CO1) gene demonstrated that the species is Spirometra erinaceieuropaei. Regarding optimum climate conditions and previous reports of animal infection in this region, the likelihood of human involvement should be potentially considered.
人兽共患寄生虫裂头绦虫属有多个物种，分布几乎遍及全球。在此，我们描述了在伊朗一只被车撞死的野猫（欧洲野猫）中首次对猬迭宫绦虫进行详细的分子检测，并使用细胞色素c氧化酶亚基I（CO1）基因在物种水平上对其进行鉴定。采用CTAB提取法提取基因组DNA。然后将该DNA用于细胞色素c氧化酶亚基I（CO1）基因的PCR扩增。之后，对PCR产物进行测序，并使用BLAST程序、ClustalX和Bioedit软件对获得的数据进行分析和多重比对。显微镜检查结果和诊断线索显示该寄生虫是一种裂头绦虫属绦虫。因此，基于细胞色素c氧化酶亚基1（CO1）基因的分子分析表明该物种为猬迭宫绦虫。鉴于该地区的最佳气候条件以及此前动物感染的报告，应潜在地考虑人类感染的可能性。
