Patra Gautam, Borthakur Sonjoy Kumar, Das Samares, Lalliankimi Hmar, Lalrinkima Hniang
Department of Veterinary Parasitology, College of Veterinary Sciences and Animal Husbandry, Selesih, Aizawl, India.
Department of Veterinary Parasitology, College of Veterinary Sciences and Animal Husbandry, Selesih, Aizawl, India.
Vet Parasitol Reg Stud Reports. 2017 May;8:99-103. doi: 10.1016/j.vprsr.2017.03.002. Epub 2017 Mar 9.
To evaluate the role of IL-13 gene in Garole/Sahabadi sheep against resistant/susceptibility to Haemonchus contortus five Garole sheep (Group I) with consistently showing low egg per gram of faeces (EPG) (≤150) and another five Garole sheep (Group II) with high EPG (≥500) were selected and fed orally with active H. contortus third stage (L) larvae at the dose rate of 500 larvae/kg body weight. Five Sahabadi sheep (Gr-III) known susceptible to H. contortus were also challenged with the same dose as the previous groups. Blood was collected from each group on day 0, 7 and 14days post infection (dpi). The serum level of IL-13 cytokine was evaluated by ELISA at different days of post infection. The ovine IL-13 was partially isolated and the RNAm level of IL-13 expression was studied by real-time PCR at different days post infection. In the resistant Garole sheep (Gr-I) the concentration of IL-13 cytokine increased significantly (P≤0.05) 14days PI compared to susceptible Garole (Gr-II)/Sahabadi (Group-III). In similar way the relative expression of IL-13 in group I (Gr-I) was found more than seven fold higher than Gr-II and Gr-III on 14 dpi. The Th type immune response to H. contortus characterized by higher serum level of IL-13 cytokine and IL-13 gene expression indicated that IL-13 gene plays a vital role for the reduced establishment and/or survival of established H. contortus in resistant Garole sheep.
为了评估白细胞介素-13（IL-13）基因在加罗尔/萨哈巴迪绵羊抵抗捻转血矛线虫感染中的作用，选择了五只粪便虫卵计数（EPG）一直较低（≤150）的加罗尔绵羊（第一组）和另外五只EPG较高（≥500）的加罗尔绵羊（第二组），并以每千克体重500条感染性捻转血矛线虫第三期幼虫（L3）的剂量经口投喂。五只已知对捻转血矛线虫易感的萨哈巴迪绵羊（第三组）也接受与前几组相同剂量的感染。在感染后第0、7和14天从每组采集血液。通过酶联免疫吸附测定（ELISA）评估感染后不同时间点血清中IL-13细胞因子的水平。对绵羊IL-13进行了部分分离，并通过实时聚合酶链反应（PCR）研究感染后不同时间点IL-13表达的RNA水平。与易感的加罗尔绵羊（第二组）/萨哈巴迪绵羊（第三组）相比，在抗捻转血矛线虫的加罗尔绵羊（第一组）中，感染后14天IL-13细胞因子浓度显著升高（P≤0.05）。同样，在感染后14天，第一组（第一组）中IL-13的相对表达量比第二组和第三组高出七倍以上。以较高的血清IL-13细胞因子水平和IL-13基因表达为特征的对捻转血矛线虫的Th型免疫反应表明，IL-13基因在抗捻转血矛线虫的加罗尔绵羊中对捻转血矛线虫感染的减少和/或已建立感染的存活起着至关重要的作用。
