Mesa-Arciniegas Paola, Parra-Henao Gabriel, Carrión-Bonifacio Ángela, Casas-Cruz Adriana, Patiño-Cuellar Ana, Díaz-Rodríguez Katherine, Garzón-Jiménez Sandra, Almansa-Manrique Jorge, Bernal-Rosas Yuly, Hernández-Lamus Claribell, Pedraza-Toscano Adriana, Torres-García Orlando
Facultad de Ciencias de la salud Programa de Bacteriología, Universidad Colegio Mayor de Cundinamarca - UCMC, Calle 28 No. 5B-02, Bogotá D.C., Colombia.
Centro de Investigación en Salud para el Trópico, Universidad Cooperativa de Colombia, Santa Marta - UCC - Troncal del Caribe, Km 3, Santa Marta, Magdalena, Colombia; Red Chagas Colombia, Cl. 26 #51-20, Bogotá D.C., Colombia.
Vet Parasitol Reg Stud Reports. 2018 Dec;14:212-216. doi: 10.1016/j.vprsr.2018.11.006. Epub 2018 Nov 28.
The seropositivity and risk factors for Trypanosoma cruzi infection in dogs from a municipality of Cundinamarca, a central state of Colombia were studied. A total of 356 client-owned dogs from urban, peri-urban and rural areas of La Mesa municipality, (Cundinamarca, Colombia) were randomly selected. Blood samples were collected by venipuncture. Anti-T. cruzi antibodies were determined using the enzyme-linked immunosorbent assay (ELISA) method. Reactive ELISA sera were processed by indirect immunofluorescence to confirm the presence of anti-T. cruzi antibodies. Chi-square tests were conducted for statistical analysis. Serologic tests for T. cruzi infection showed a prevalence of 29.49% (105/356), the rural area show a highest T. cruzi infection pattern in comparison with the other zone locations. Two triatomine species were found through the study: Panstrongylus geniculatus (53.4%) and Rhodnius colombiensis (46.6%). The prevalence of positive vectors for parasite was of 52.1% (38/73). Additionally, a very close relation between triatomine bugs and dogs in the rural zone (1:3.1) was observed. These results are the first report of natural infection by T. cruzi in domestic dogs in La Mesa municipality. In conclusion, the presence of anti-T. cruzi antibodies in dogs in this area suggest vector transmission. There is a need for active surveillance programs throughout the La Mesa municipality and vector control strategies should also be implemented.
对哥伦比亚中部昆迪纳马卡省一个市镇的犬类克氏锥虫感染的血清阳性率及风险因素进行了研究。从哥伦比亚昆迪纳马卡省拉梅萨市的城市、城郊和农村地区随机选取了356只客户拥有的犬只。通过静脉穿刺采集血样。采用酶联免疫吸附测定（ELISA）法检测抗克氏锥虫抗体。对ELISA反应性血清进行间接免疫荧光处理，以确认抗克氏锥虫抗体的存在。采用卡方检验进行统计分析。克氏锥虫感染的血清学检测显示患病率为29.49%（105/356），与其他区域相比，农村地区克氏锥虫感染模式最高。通过研究发现了两种锥蝽：膝状锥蝽（53.4%）和哥伦比亚红猎蝽（46.6%）。寄生虫阳性媒介的患病率为52.1%（38/73）。此外，在农村地区观察到锥蝽与犬之间存在非常密切的关系（1:3.1）。这些结果是拉梅萨市家犬自然感染克氏锥虫的首次报告。总之，该地区犬类中抗克氏锥虫抗体的存在表明存在媒介传播。需要在整个拉梅萨市开展积极的监测计划，还应实施媒介控制策略。
