Vieira L Q, Colley D G, de Souza C P, Gazzinelli G
Am J Trop Med Hyg. 1987 Jan;36(1):83-91. doi: 10.4269/ajtmh.1987.36.83.
Several antigenic preparations derived from Schistosoma mansoni schistosomula were compared as immunogens in proliferation assays utilizing peripheral blood mononuclear cells (PBMN) from patients with chronic intestinal schistosomiasis mansoni. Living and fixed schistosomula, either irradiated or nonirradiated, and freshly transformed or 24 hr cultured worms were found to induce very similar proliferative responses of patients' PBMN. Two schistosomulum surface membrane preparations were also compared. Membrane vesicles from schistosomula were found to elicit mean proliferative responses similar to those stimulated by a CaCl2-extracted tegument preparation and to do so at lower total protein concentrations. However, the responses to membrane vesicles were somewhat more variable. When saline extracts from schistosomular bodies submitted to these two treatments were used as stimulants in parallel PBMN cultures it was found that vesiculation more effectively removed antigens involved in the stimulation of PBMN. Broken vesicles were less stimulatory than intact ones. This suggests that the manner in which membrane antigens are presented to antigen processing cells is critical. Incubation of schistosomula at 37 degrees C, but not 4 degrees C in medium containing 10% fetal calf serum from 3 to 48 hr decreased the capacity of the membrane to produce vesicles when treated with high salt solution.
在利用慢性曼氏血吸虫病患者外周血单个核细胞（PBMN）进行的增殖试验中，比较了几种源自曼氏血吸虫童虫的抗原制剂作为免疫原的效果。活的和固定的童虫，无论是否经过辐照，以及刚转化的或培养24小时的虫体，都能诱导患者PBMN产生非常相似的增殖反应。还比较了两种童虫表面膜制剂。发现来自童虫的膜泡引发的平均增殖反应与氯化钙提取的皮层制剂刺激的反应相似，并且在较低的总蛋白浓度下就能做到。然而，对膜泡的反应在一定程度上更具变异性。当将经过这两种处理的童虫体生理盐水提取物作为平行PBMN培养中的刺激物时，发现囊泡化更有效地去除了参与刺激PBMN的抗原。破碎的囊泡比完整的囊泡刺激性小。这表明膜抗原呈递给抗原处理细胞的方式至关重要。在含有10%胎牛血清的培养基中于37℃而非4℃孵育童虫3至48小时，会降低用高盐溶液处理时膜产生囊泡的能力。
