Lechat P, Eugène M, Hadjiisky P, Teillac A, Cabrol C, Grosgogeat Y
Arch Mal Coeur Vaiss. 1986 Aug;79(9):1356-60.
Rejection of cardiac transplants can be detected by NMR imaging if it is associated with a change in myocardial T1 and/or T2 proton relaxation time. T1 and T2 were studied in 14 Lewis rats that underwent heterotopic cardiac transplantation. T1 and T2 were measured in vitro immediately after sacrifice 3, 4, 7 or 11 days after the graft using a Minispec BRUKER PC20. The myocardial water content was measured by dehydration in a vacuum for 24 hours. Histological analysis of sections classified the rejection process in 4 stages according to the degree of lymphocyte infiltration and percentage of myolysis. There was a significant difference between the ortho and heterotopic hearts: (Formula: see text). In particular, there was highly significant relationship between T2 and the stage of rejection (r = 0.90, p less than 0.005), and between T2 and % myolysis (r = 0.84, p less than 0.005). In addition, there was a close relationship between the T2 of the ortho and heterotopic hearts and their water content (r = 0.95, p less than 0.001). If these results are confirmed in man, is should be possible to detect rejection by NMR imaging using sequences of activation concentrating on changes of T2.
如果心脏移植排斥反应与心肌T1和/或T2质子弛豫时间的变化相关，那么通过核磁共振成像（NMR成像）就能检测到。对14只接受异位心脏移植的Lewis大鼠的T1和T2进行了研究。在移植后3、4、7或11天处死后，立即使用Minispec BRUKER PC20在体外测量T1和T2。通过在真空中脱水24小时来测量心肌含水量。根据淋巴细胞浸润程度和肌溶解百分比，对切片进行组织学分析，将排斥过程分为4个阶段。原位心脏和异位心脏之间存在显著差异：（公式：见正文）。特别是，T2与排斥阶段之间存在高度显著的关系（r = 0.90，p小于0.005），T2与肌溶解百分比之间也存在高度显著的关系（r = 0.84，p小于0.005）。此外，原位心脏和异位心脏的T2与其含水量之间存在密切关系（r = 0.95，p小于0.001）。如果这些结果在人体中得到证实，那么使用聚焦于T2变化的激活序列，通过NMR成像检测排斥反应应该是可行的。
