Eugene M, Lechat P, Hadjiisky P, Teillac A, Grosgogeat Y, Cabrol C
J Heart Transplant. 1986 Jan-Feb;5(1):39-45.
It should be possible to detect heart transplant rejection by nuclear magnetic resonance (NMR) imaging if it induces myocardial T1 and T2 proton relaxation time alterations or both. We studied 20 Lewis rats after a heterotopic heart transplantation. In vitro measurement of T1 and T2 was performed on a Minispec PC20 (Bruker) 3 to 9 days after transplantation. Histologic analysis allowed the quantification of rejection process based on cellular infiltration and myocardiolysis. Water content, a major determinant of relaxation time, was also studied. T1 and T2 were significantly prolonged in heterotopic vs orthotopic hearts (638 +/- 41 msec vs 606 +/- 22 msec for T1, p less than 0.01 and 58.2 +/- 8.4 msec vs 47.4 +/- 1.9 msec for T2, p less than 0.001). Water content was also increased in heterotopic hearts (76.4 +/- 2.3 vs 73.8 +/- 1.0, p less than 0.01). Most importantly, we found close correlations between T1 and especially T2 vs water content, cellular infiltration, and myocardiolysis. We conclude that rejection reaction should be noninvasively detected by NMR imaging, particularly with pulse sequences emphasizing T2.
如果心脏移植排斥反应能引起心肌T1和T2质子弛豫时间的改变或两者皆有改变，那么通过核磁共振（NMR）成像检测心脏移植排斥反应应该是可行的。我们对20只进行了异位心脏移植的Lewis大鼠进行了研究。在移植后3至9天，使用Minispec PC20（布鲁克公司）对T1和T2进行体外测量。组织学分析可以根据细胞浸润和心肌溶解情况对排斥反应过程进行量化。还研究了作为弛豫时间主要决定因素的含水量。异位心脏与原位心脏相比，T1和T2显著延长（T1：638±41毫秒对606±22毫秒，p<0.01；T2：58.2±8.4毫秒对47.4±1.9毫秒，p<0.001）。异位心脏的含水量也增加了（76.4±2.3对73.8±1.0，p<0.01）。最重要的是，我们发现T1尤其是T2与含水量、细胞浸润和心肌溶解之间存在密切相关性。我们得出结论，排斥反应应该可以通过NMR成像进行无创检测，特别是使用强调T2的脉冲序列。
