内细胞团干细胞的异质性。

Heterogeneity in Epiblast Stem Cells.

机构信息

UMR BDR, INRA, ENVA, Université Paris Saclay, Jouy en Josas, France.

出版信息

Adv Exp Med Biol. 2019;1123:5-17. doi: 10.1007/978-3-030-11096-3_2.

Abstract

Epiblast stem cells (EpiSCs) are pluripotent cells that are derived from mouse embryos at gastrulation stages. They represent the primed state of pluripotency, in which cells are on the verge of differentiation and already express markers of the three primary lineages (mesoderm, endoderm, neurectoderm). EpiSCs display some heterogeneity intra- and inter-cell lines in the expression of some of these lineage markers. We relate this heterogeneity to signalling pathways that are active in EpiSCs, either due to addition of growth factors (FGF2 and activin) in the culture medium, or endogenously active (FGF, Nodal, and Wnt). By modulating Wnt or activin/nodal pathways, cell lines close to EpiSCs but with different properties can be obtained. These signalling pathways are all at work in vivo to pattern the pluripotent epiblast and specify cellular fates.

摘要

内细胞团干细胞（EpiSCs）是多能细胞，来源于原肠胚期的小鼠胚胎。它们代表了多能性的初始状态，此时细胞处于分化的边缘，已经表达了三个主要谱系（中胚层、内胚层和神经外胚层）的标志物。EpiSCs 在表达其中一些谱系标志物方面存在细胞内和细胞间的异质性。我们将这种异质性与 EpiSCs 中活跃的信号通路联系起来，这些信号通路要么是由于培养基中添加了生长因子（FGF2 和激活素），要么是内源性活跃的（FGF、Nodal 和 Wnt）。通过调节 Wnt 或激活素/ Nodal 通路，可以获得接近 EpiSCs 但具有不同特性的细胞系。这些信号通路在体内都在发挥作用，以对多能性内细胞团进行模式化并指定细胞命运。