Stewart B W, Haber M, Ward E J
Biochem Int. 1986 Nov;13(5):903-13.
The proportion of sheared rat liver DNA recovered from benzoylated DEAE-cellulose in the final stage following stepwise elution with NaCl and caffeine solutions was dependent upon the DNA isolation procedure. An increase in the proportion of DNA containing single stranded regions, consequent upon delay or addition of Mg2+ prior to phenol extraction, suggested nuclease mediated degradation. Administration of methyl methanesulphonate to rats resulted in a consistent proportional increase in the caffeine-eluted fraction. The results of caffeine gradient elution of control and alkylated DNA from benzoylated DEAE-cellulose were consistent with repair-associated single stranded regions being substrates for endogenous single strand-specific exonucleases.
在用氯化钠和咖啡因溶液逐步洗脱后的最后阶段，从苯甲酰化二乙氨基乙基纤维素中回收的剪切大鼠肝脏DNA的比例取决于DNA分离程序。在酚提取之前延迟或添加镁离子后，含单链区域的DNA比例增加，提示核酸酶介导的降解。给大鼠施用甲基磺酸甲酯导致咖啡因洗脱部分一致地成比例增加。从苯甲酰化二乙氨基乙基纤维素中对对照和烷基化DNA进行咖啡因梯度洗脱的结果与修复相关的单链区域作为内源性单链特异性核酸外切酶的底物一致。
