Colten H R, Dowton S B
Biochem Soc Symp. 1986;51:37-46.
The availability of molecular probes for approximately half of the 20 known complement genes now permits a detailed examination of the regulation of complement expression in liver and at extrahepatic sites in tissue macrophages. Primary cell culture, cell lines and cells transfected with DNA bearing complement genes have been used in this analysis. Pretranslational regulation of complement production has been induced by well defined cytokines such as interleukin-1 and gamma-interferon, as well as directly by endotoxin. The effect of those agents on complement genes is tissue and species specific and is developmentally regulated. These data form the basis for the elucidation of the genomic structural requirements for regulation of inflammation and, by extension, specific immune responsiveness.
目前，针对20种已知补体基因中约一半的分子探针已可获取，这使得详细研究肝脏及组织巨噬细胞肝外部位补体表达的调控成为可能。原代细胞培养、细胞系以及用携带补体基因的DNA转染的细胞已用于此项分析。补体产生的翻译前调控已由明确的细胞因子如白细胞介素-1和γ-干扰素诱导产生，也可由内毒素直接诱导。这些因子对补体基因的作用具有组织和物种特异性，且受发育调控。这些数据为阐明炎症调控以及由此延伸的特异性免疫反应性的基因组结构要求奠定了基础。
