头颈部组织驻留间充质干细胞的辐射敏感性。
Radiation-induced sensitivity of tissue-resident mesenchymal stem cells in the head and neck region.
机构信息
Department of Otorhinolaryngology, Klinikum der Universitaet Muenchen, Ludwig-Maximilians-Universitaet Muenchen, Munich, Germany.
Department of Otorhinolaryngology, Universitaetsmedizin Goettingen, University Medical Center Goettingen, Goettingen, Germany.
出版信息
Head Neck. 2019 Sep;41(9):2892-2903. doi: 10.1002/hed.25768. Epub 2019 Apr 24.
BACKGROUND
Tissue-resident mesenchymal stem cells (MSCs) possess the ability to migrate to areas of inflammation and promote the regeneration of damaged tissue. However, it remains unclear how radiation influences this capacity of MSC in the head and neck region.
METHODS
Two types of MSCs of the head and neck region (mucosa [mMSC] and parotid gland [pMSC]) were isolated, cultured and exposed to single radiation dosages of 2 Gy/day up to 10 days. Effects on morphology, colony forming ability, apoptosis, chemokine receptor expression, cytokine secretion, and cell migration were analyzed.
RESULTS
Although MSC preserved MSC-specific regenerative abilities and immunomodulatory properties following irradiation in our in vitro model, we found a deleterious impact on colony forming ability, especially in pMSC.
CONCLUSIONS
MSC exhibited robustness and activation upon radiation for the support of tissue regeneration, but lost their potential to replicate, thus possibly leading to depletion of the local MSC-pool after irradiation.
背景
组织驻留间充质干细胞（MSCs）具有迁移至炎症区域并促进受损组织再生的能力。然而，目前尚不清楚辐射会如何影响头颈部 MSC 的这种能力。
方法
分离并培养头颈部两种类型的 MSC（黏膜[mMSC]和腮腺[pMSC]），并使其每天接受 2 Gy 的单次辐射剂量，持续 10 天。分析形态、集落形成能力、细胞凋亡、趋化因子受体表达、细胞因子分泌和细胞迁移的影响。
结果
尽管我们的体外模型中的 MSC 在辐照后保留了 MSC 特异性的再生能力和免疫调节特性，但我们发现集落形成能力受到了有害影响，尤其是在 pMSC 中。
结论
MSC 在辐射支持组织再生时表现出稳健性和激活性，但失去了复制的潜力，因此可能会导致照射后局部 MSC 池的耗竭。
Zotero 插件