Hughes R G, Neill W A, Norval M
Br Med J (Clin Res Ed). 1987 Jan 31;294(6567):267-9. doi: 10.1136/bmj.294.6567.267.
Cervical biopsy samples were taken from 79 patients who had various grades of cervical intraepithelial neoplasia or who showed evidence, in the form of koilocytosis, of human papillomavirus infection of the uterine cervix and from 10 women with normal cervices. The DNA content of the cells in the samples was analysed by flow cytometry. Analysis of the data obtained showed that the biopsy samples from women with cervical intraepithelial neoplasia and human papillomavirus lesions contained significantly more dividing cells (31.2% of cells from human papillomavirus lesions with no cervical intraepithelial neoplasia and 33.06%, 29.89%, and 31.76% of cells from cervical intraepithelial neoplasia grades I, II, and III, respectively) than those from women with normal cervices (21.6%). The proportion of aneuploid samples from the group who showed evidence of human papillomavirus infection only (18.2%) did not differ significantly from the group with cervical intraepithelial neoplasia grade III (21.2%). Aneuploidy and an increased rate of cellular proliferation are recognised characteristics of malignancy. These results therefore support the view that human papillomavirus plays an important part in the aetiology of cervical carcinoma and are relevant to the clinical management of patients.
从79例患有不同级别的宫颈上皮内瘤变或表现出挖空细胞形式的人乳头瘤病毒子宫颈感染证据的患者以及10名宫颈正常的女性身上采集了宫颈活检样本。通过流式细胞术分析样本中细胞的DNA含量。对所得数据的分析表明，患有宫颈上皮内瘤变和人乳头瘤病毒病变的女性的活检样本中，分裂细胞显著多于宫颈正常女性的活检样本（仅有人乳头瘤病毒病变而无宫颈上皮内瘤变的样本中31.2%的细胞，以及宫颈上皮内瘤变I级、II级和III级样本中分别为33.06%、29.89%和31.76%的细胞，而宫颈正常女性的样本中为21.6%）。仅显示人乳头瘤病毒感染证据的组中非整倍体样本的比例（18.2%）与宫颈上皮内瘤变III级组（21.2%）无显著差异。非整倍体和细胞增殖率增加是恶性肿瘤公认的特征。因此，这些结果支持人乳头瘤病毒在子宫颈癌病因学中起重要作用的观点，并且与患者的临床管理相关。
