Reddy A L, Caldwell M, Fialkow P J
Cancer Res. 1987 Apr 1;47(7):1947-51.
Most mouse skin papillomas induced by 7,12-dimethylbenz(a)-anthracene initiation followed by 12-O-tetradecanoylphorbol-13 acetate (TPA) promotion are benign promoter-dependent papillomas which regress after cessation of promotion, but some benign tumors (promoter-independent papillomas) do not regress, and a few carcinomas seem to develop from progressive growth of these tumors. We have tested whether a second course of TPA promotion induces regeneration in regressed promoter-dependent papillomas and advances them to malignancy. The regression and regeneration of these papillomas were determined by serial photographs, measurements of coordinates, histopathological evaluation, and X-chromosome-linked phosphoglycerate kinase enzyme cellular markers. Most of the regressed promoter-dependent papillomas did not regenerate. However, the second course of TPA promotion induced rapid development of many new papillomas, some of which advanced to promoter-independent papillomas and a carcinoma. This finding suggests that there are more abnormal cells in the initiated mouse skin than those detected with a single course of TPA promotion.
大多数由7,12-二甲基苯并(a)蒽引发、随后用12-O-十四烷酰佛波醇-13-乙酸酯（TPA）促癌的小鼠皮肤乳头瘤是良性的促癌依赖性乳头瘤，在停止促癌后会消退，但一些良性肿瘤（促癌非依赖性乳头瘤）不会消退，少数癌似乎由这些肿瘤的渐进性生长发展而来。我们测试了第二轮TPA促癌是否会诱导已消退的促癌依赖性乳头瘤再生，并使其发展为恶性肿瘤。这些乳头瘤的消退和再生通过系列照片、坐标测量、组织病理学评估以及X染色体连锁磷酸甘油酸激酶酶细胞标志物来确定。大多数已消退的促癌依赖性乳头瘤没有再生。然而，第二轮TPA促癌诱导了许多新乳头瘤的快速形成，其中一些发展为促癌非依赖性乳头瘤和一种癌。这一发现表明，在起始的小鼠皮肤中存在比单次TPA促癌检测到的更多异常细胞。
