Predicting RNA Families in Nucleotide Sequences Using StructRNAfinder.

Noncoding RNA (ncRNA) research is already a routine in every genomics or transcriptomics initiatives. According to their functions, ncRNAs can be grouped into several different RNA families, which can be represented by conserved primary sequences, secondary structures, or covariance models (CMs). CMs are very sensitive in predicting RNA families in nucleotide sequences and have been widely used in characterizing the repertoire of ncRNAs in organisms from all domains of life. However, the large-scale prediction and annotation of ncRNAs require multiple tools along the process, imposing a great obstacle for researchers with lesser computational or bioinformatics background. StructRNAfinder emerged as an automated tool to avoid these bottlenecks, by performing the automatic identification and complete annotation of regulatory RNA families derived directly from nucleotide sequences. In this chapter, we provide a complete tutorial for both stand-alone and web server versions of StructRNAfinder. This will help users to install the tool and to perform predictions of RNA families in any genome or transcriptome sequences dataset.