通过近红外荧光蛋白（iRFP）标记对缺血性心脏中的干细胞植入进行成像和跟踪。
Imaging and Tracking Stem Cell Engraftment in Ischemic Hearts by Near-Infrared Fluorescent Protein (iRFP) Labeling.
机构信息
Vascular Biology Center, Medical College of Georgia, Augusta University, Augusta, GA, USA.
出版信息
Methods Mol Biol. 2020;2150:121-129. doi: 10.1007/7651_2019_226.
Abstract
Stem cell-based therapies hold great promise as alternative therapeutic strategies for various chronic diseases, including ischemic cardiomyopathy. Tracking the engraftment of transplanted stem cells is critical to the assessment of donor cell survival in the host environment. Fluorescent proteins, such as green fluorescent protein (GFP), have been widely used to track the fate of donor cells; however, GFP labeling has limitations with regard to noninvasively measuring cell engraftment in vivo. Our research indicates that near-infrared fluorescent protein (iRFP) labeling offers advantages for noninvasive in vivo imaging and histological assessment. Here, we present a protocol for using the lentiviral vector-mediated iRFP vector system to label and track donor stem cells in ischemic mouse hearts.
摘要
基于干细胞的疗法作为各种慢性疾病的替代治疗策略具有很大的潜力，包括缺血性心肌病。跟踪移植干细胞的植入对于评估供体细胞在宿主环境中的存活至关重要。荧光蛋白，如绿色荧光蛋白（GFP），已被广泛用于跟踪供体细胞的命运；然而，GFP 标记在非侵入性地测量体内细胞植入方面存在局限性。我们的研究表明，近红外荧光蛋白（iRFP）标记在非侵入性体内成像和组织学评估方面具有优势。在这里，我们提出了一种使用慢病毒载体介导的 iRFP 载体系统标记和跟踪缺血性小鼠心脏中的供体干细胞的方案。
相似文献
1
Imaging and Tracking Stem Cell Engraftment in Ischemic Hearts by Near-Infrared Fluorescent Protein (iRFP) Labeling.
Methods Mol Biol. 2020;2150:121-129. doi: 10.1007/7651_2019_226.
2
Assessing in vitro stem-cell function and tracking engraftment of stem cells in ischaemic hearts by using novel iRFP gene labelling.
J Cell Mol Med. 2014 Sep;18(9):1889-94. doi: 10.1111/jcmm.12321. Epub 2014 Jun 9.
3
AAV-iRFP labelling of human mesenchymal stem cells for near-infrared fluorescence imaging.
Biosci Rep. 2017 Apr 28;37(2). doi: 10.1042/BSR20160556. Print 2017 Apr 30.
4
Using iRFP Genetic Labeling Technology to Track Tumorogenesis of Transplanted CRISPR/Cas9-Edited iPSC in Skeletal Muscle.
Methods Mol Biol. 2020;2126:73-83. doi: 10.1007/978-1-0716-0364-2_7.
5
Infrared fluorescent protein 1.4 genetic labeling tracks engrafted cardiac progenitor cells in mouse ischemic hearts.
PLoS One. 2014 Oct 30;9(10):e107841. doi: 10.1371/journal.pone.0107841. eCollection 2014.
6
Identification of stem cells after transplantation.
Methods Mol Biol. 2013;1036:89-94. doi: 10.1007/978-1-62703-511-8_8.
7
Tracking and Imaging of Transplanted Stem Cells in Animals.
Methods Mol Biol. 2020;2150:45-56. doi: 10.1007/7651_2019_275.
8
Dynamic tracking of stem cells in an acute liver failure model.
World J Gastroenterol. 2012 Feb 14;18(6):507-16. doi: 10.3748/wjg.v18.i6.507.
9
Assessment of biological characteristics of adipose tissue-derived stem cells co-labeled with Molday ION Rhodamine B™ and green fluorescent protein in vitro.
Mol Med Rep. 2013 Nov;8(5):1446-52. doi: 10.3892/mmr.2013.1694. Epub 2013 Sep 18.
10
Recent Advances in Tracking the Transplanted Stem Cells Using Near-Infrared Fluorescent Nanoprobes: Turning from the First to the Second Near-Infrared Window.
Adv Healthc Mater. 2018 Oct;7(20):e1800497. doi: 10.1002/adhm.201800497. Epub 2018 Jul 17.
引用本文的文献
1
Restoration of Dystrophin Expression in Mdx-Derived Muscle Progenitor Cells Using CRISPR/Cas9 System and Homology-Directed Repair Technology.
Methods Mol Biol. 2023;2587:455-464. doi: 10.1007/978-1-0716-2772-3_23.
2
Establishment and characterization of a novel human induced pluripotent stem cell line stably expressing the iRFP720 reporter.
Sci Rep. 2022 Jun 14;12(1):9874. doi: 10.1038/s41598-022-12956-1.
3
Nanoparticles for Stem Cell Tracking and the Potential Treatment of Cardiovascular Diseases.
Front Cell Dev Biol. 2021 Jul 2;9:662406. doi: 10.3389/fcell.2021.662406. eCollection 2021.
4
3D Printing and NIR Fluorescence Imaging Techniques for the Fabrication of Implants.
Materials (Basel). 2020 Oct 28;13(21):4819. doi: 10.3390/ma13214819.
5
The Small GTPases Rab27b Regulates Mitochondrial Fatty Acid Oxidative Metabolism of Cardiac Mesenchymal Stem Cells.
Front Cell Dev Biol. 2020 Apr 15;8:209. doi: 10.3389/fcell.2020.00209. eCollection 2020.
本文引用的文献
1
Transplantation of Cardiac Mesenchymal Stem Cell-Derived Exosomes for Angiogenesis.
J Cardiovasc Transl Res. 2018 Oct;11(5):429-437. doi: 10.1007/s12265-018-9824-y. Epub 2018 Oct 1.
2
Transplantation of Cardiac Mesenchymal Stem Cell-Derived Exosomes Promotes Repair in Ischemic Myocardium.
J Cardiovasc Transl Res. 2018 Oct;11(5):420-428. doi: 10.1007/s12265-018-9822-0. Epub 2018 Sep 19.
3
Exosome-Derived Dystrophin from Allograft Myogenic Progenitors Improves Cardiac Function in Duchenne Muscular Dystrophic Mice.
J Cardiovasc Transl Res. 2018 Oct;11(5):412-419. doi: 10.1007/s12265-018-9826-9. Epub 2018 Aug 28.
4
Recent Advances in Tracking the Transplanted Stem Cells Using Near-Infrared Fluorescent Nanoprobes: Turning from the First to the Second Near-Infrared Window.
Adv Healthc Mater. 2018 Oct;7(20):e1800497. doi: 10.1002/adhm.201800497. Epub 2018 Jul 17.
5
Regenerative Therapy for Cardiomyopathies.
J Cardiovasc Transl Res. 2018 Oct;11(5):357-365. doi: 10.1007/s12265-018-9807-z. Epub 2018 May 9.
6
Infrared fluorescent protein 1.4 genetic labeling tracks engrafted cardiac progenitor cells in mouse ischemic hearts.
PLoS One. 2014 Oct 30;9(10):e107841. doi: 10.1371/journal.pone.0107841. eCollection 2014.
7
Assessing in vitro stem-cell function and tracking engraftment of stem cells in ischaemic hearts by using novel iRFP gene labelling.
J Cell Mol Med. 2014 Sep;18(9):1889-94. doi: 10.1111/jcmm.12321. Epub 2014 Jun 9.
8
Identification of stem cells after transplantation.
Methods Mol Biol. 2013;1036:89-94. doi: 10.1007/978-1-62703-511-8_8.
9
The worldwide environment of cardiovascular disease: prevalence, diagnosis, therapy, and policy issues: a report from the American College of Cardiology.
J Am Coll Cardiol. 2012 Dec 25;60(25 Suppl):S1-49. doi: 10.1016/j.jacc.2012.11.002.
10
An infrared fluorescent protein for deeper imaging.
Nat Biotechnol. 2011 Aug 5;29(8):715-6. doi: 10.1038/nbt.1941.
Zotero 插件