移植后干细胞的鉴定。
Identification of stem cells after transplantation.
作者信息
Wang Yingjie, Zhang Lan, Pan Yaohua, Chen Lijuan, Weintraub Neal, Tang Yaoliang
机构信息
Department of Internal Medicine, Division of Cardiovascular Disease, College of Medicine, University of Cincinnati, Cincinnati, OH, USA.
出版信息
Methods Mol Biol. 2013;1036:89-94. doi: 10.1007/978-1-62703-511-8_8.
Abstract
The ability to identify the donor stem cells following transplantation into injured hearts is critical. This is particularly important in evaluating stem cell survival and lineage differentiation into mature cardiovascular cells. Several approaches have been employed for tracking the donor stem cells, including fluorescent dyes and fluorescent protein gene transfer. Here, we will induce a protocol using lentivirus-mediated green fluorescent protein (GFP) to monitor the fate of donor stem cells following transplantation.
摘要
移植到受损心脏后识别供体干细胞的能力至关重要。这在评估干细胞存活以及向成熟心血管细胞的谱系分化方面尤为重要。已经采用了几种方法来追踪供体干细胞，包括荧光染料和荧光蛋白基因转移。在此，我们将采用一种方案，利用慢病毒介导的绿色荧光蛋白（GFP）来监测移植后供体干细胞的命运。
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1
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Stem Cells Dev. 2012 Aug 10;21(12):2122-9. doi: 10.1089/scd.2011.0463. Epub 2012 Feb 28.
2
Novel microRNA prosurvival cocktail for improving engraftment and function of cardiac progenitor cell transplantation.新型 microRNA 生存促进鸡尾酒，改善心脏祖细胞移植的植入和功能。
Circulation. 2011 Sep 13;124(11 Suppl):S27-34. doi: 10.1161/CIRCULATIONAHA.111.017954.
3
PKH26 as a fluorescent label for live human umbilical mesenchymal stem cells.PKH26 作为活的人脐带间充质干细胞的荧光标记物。
In Vitro Cell Dev Biol Anim. 2011 Sep;47(8):516-20. doi: 10.1007/s11626-011-9424-5. Epub 2011 Jul 30.
4
Hypoxic preconditioning enhances the benefit of cardiac progenitor cell therapy for treatment of myocardial infarction by inducing CXCR4 expression.缺氧预处理通过诱导CXCR4表达增强心脏祖细胞治疗心肌梗死的益处。
Circ Res. 2009 May 22;104(10):1209-16. doi: 10.1161/CIRCRESAHA.109.197723. Epub 2009 Apr 30.
5
A novel two-step procedure to expand cardiac Sca-1+ cells clonally.一种克隆扩增心脏Sca-1+细胞的新型两步法。
Biochem Biophys Res Commun. 2007 Aug 10;359(4):877-83. doi: 10.1016/j.bbrc.2007.05.216. Epub 2007 Jun 11.
6
Restoration of cardiac progenitor cells after myocardial infarction by self-proliferation and selective homing of bone marrow-derived stem cells.心肌梗死后通过骨髓源性干细胞的自我增殖和选择性归巢实现心脏祖细胞的恢复。
Circ Res. 2005 Nov 25;97(11):1090-2. doi: 10.1161/01.RES.0000194330.66545.f5. Epub 2005 Nov 3.
7
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Regul Pept. 2004 Jan 15;117(1):3-10. doi: 10.1016/j.regpep.2003.09.005.
8
Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector.在注射慢病毒载体的成年大鼠脑中，转基因的高效转移、整合及长期持续表达。
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9
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10
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