Maruo F, Okada M
Cell Differ. 1987 Jan;20(1):45-54. doi: 10.1016/0045-6039(87)90464-7.
A library of monoclonal antibodies (MAbs) against Drosophila ovarian antigens was established. Each of the MAbs was characterized by its immunohistochemical binding pattern to sections from egg chambers at various stages of oogenesis. Sixteen of the 18 MAbs were found to bind to antigens in mature oocytes. Among the 16 antigens, two were also located in cytoplasm of cell types in the egg chamber other than the oocyte, at all stages of oogenesis. Four made their appearance in nurse cell cytoplasm at mid-vitellogenic stages and shifted to oocyte cytoplasm at a later stage, and ten appeared at the vitellogenic stage and confined their distribution to oocyte cytoplasm. All these antigens were distributed evenly in cytoplasm of mature oocytes. However, some of these antigens were noticed to change their distribution during early embryogenesis as to be localized in a specific region of embryos.
建立了一个针对果蝇卵巢抗原的单克隆抗体（MAb）文库。通过免疫组织化学方法，研究了每种MAb与处于卵子发生不同阶段的卵室切片的结合模式。在18种MAb中，有16种被发现能与成熟卵母细胞中的抗原结合。在这16种抗原中，有两种在卵子发生的所有阶段，也存在于卵室中除卵母细胞外的其他细胞类型的细胞质中。四种抗原在卵黄发生中期出现在滋养细胞的细胞质中，后期转移到卵母细胞的细胞质中，十种抗原在卵黄发生期出现，并局限于卵母细胞的细胞质中。所有这些抗原在成熟卵母细胞的细胞质中均匀分布。然而，其中一些抗原在早期胚胎发育过程中分布发生了变化，定位于胚胎的特定区域。
