Egawa S E, Abo T, Hiwatashi N
Cell Immunol. 1987 Feb;104(2):386-99. doi: 10.1016/0008-8749(87)90040-2.
The Fc receptor for IgG on human natural killer (NK) cells can be identified by a series of murine monoclonal antibodies. When these antibodies with IgG1 isotype (e.g., Leu-11a and Leu-11c), but not with IgM (e.g., Leu-11b and VEP13), were added to NK assay culture against K562 targets of 4-hr analysis, a considerable enhancement of NK activity was induced. An enhancement was shown at a concentration of up to 10(-5) mg/ml of Leu-11a. Furthermore, the present experiments demonstrated that many established cell lines with diverse cell origins expressed the Leu-11 antigens, and enhancement of NK activity by Leu-11 was induced when these Leu-11+ cell lines were used as targets in NK assays. The results that Leu-11a caused an increase in effector-to-target cell conjugates, and that F(ab')2 of Leu-11a was effective in enhancement of NK activity, but the Fab was not, indicated that Leu-11 might become a linkage between the effector and target cells. Significance of this phenomenon was discussed regarding practical application of Leu-11 antibodies in laboratory experiments and in clinical studies.
人自然杀伤（NK）细胞上的IgG Fc受体可通过一系列鼠单克隆抗体来识别。当将这些具有IgG1同种型的抗体（例如Leu-11a和Leu-11c）而非具有IgM的抗体（例如Leu-11b和VEP13）添加到针对K562靶标的4小时分析的NK检测培养物中时，可诱导NK活性显著增强。在Leu-11a浓度高达10(-5) mg/ml时显示出增强作用。此外，本实验表明，许多具有不同细胞来源的已建立细胞系表达Leu-11抗原，并且当这些Leu-11+细胞系在NK检测中用作靶标时，Leu-11可诱导NK活性增强。Leu-11a导致效应细胞与靶细胞结合物增加，以及Leu-11a的F(ab')2在增强NK活性方面有效但Fab无效的结果表明，Leu-11可能成为效应细胞与靶细胞之间的连接物。讨论了这种现象在实验室实验和临床研究中Leu-11抗体实际应用方面的意义。
