植物复合类型的游离聚糖存在于番茄木质部汁液中。
Plant complex type free -glycans occur in tomato xylem sap.
作者信息
Tsujimori Yuta, Ogura Mikako, Rahman Md Ziaur, Maeda Megumi, Kimura Yoshinobu
机构信息
a Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University , Okayama , Japan.
b Faculty of Agriculture, Division of Agricultural Science , Okayama University , Okayama , Japan.
出版信息
Biosci Biotechnol Biochem. 2019 Jul;83(7):1310-1314. doi: 10.1080/09168451.2019.1608803. Epub 2019 Apr 25.
Free -glycans (FNGs) are ubiquitous in growing plants. Further, acidic peptide:-glycanase is believed to be involved in the production of plant complex-type FNGs (PCT-FNGs) during the degradation of dysfunctional glycoproteins. However, the distribution of PCT-FNGs in growing plants has not been analyzed. Here, we report the occurrence of PCT-FNGs in the xylem sap of the stem of the tomato plant. : RP-HPLC: reversed-phase HPLC; SF-HPLC: size-fractionation HPLC; PA-: pyridylamino; PCT: plant complex type; Hex: hexose; HexNAc: -acetylhexosamine; Pen: pentose; Deoxyhex: deoxyhexose; Man: D-mannose; GlcNAc: -acetyl-D-glucosamine; Xyl: D-xylose; Fuc: L-fucose; Le: Lewis a (Galβ1-3(Fucα1-4)GlcNAc); PCT: plant complex type; M3FX: Manα1-6(Manα1-3)(Xylβ1-2)Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc-PA; GN2M3FX: GlcNAcβ1-2Manα1-6(GlcNAcβ1-2Manα1-3)(Xylβ1-2)Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc-PA; (Le)1GN1M3FX: Galβ1-3(Fucα1-4)GlcNAc1-2 Manα1-6(GlcNAcβ1-2Manα1-3)(Xylβ1-2)Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc-PA or GlcNAc1-2Manα1-6(Galβ1-3(Fucα1-4)GlcNAc1-2Manα1-3)(Xylβ1-2)Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc-PA.
游离聚糖（FNGs）在生长中的植物中普遍存在。此外，酸性肽聚糖酶被认为在功能失调的糖蛋白降解过程中参与植物复合型FNGs（PCT - FNGs）的产生。然而，PCT - FNGs在生长中的植物中的分布尚未得到分析。在此，我们报道了番茄植株茎部木质部汁液中存在PCT - FNGs。：RP - HPLC：反相高效液相色谱；SF - HPLC：尺寸排阻高效液相色谱；PA -：吡啶氨基；PCT：植物复合型；Hex：己糖；HexNAc：N - 乙酰己糖胺；Pen：戊糖；Deoxyhex：脱氧己糖；Man：D - 甘露糖；GlcNAc：N - 乙酰 - D - 葡萄糖胺；Xyl：D - 木糖；Fuc：L - 岩藻糖；Le：Lewis a（Galβ1 - 3(Fucα1 - 4)GlcNAc）；PCT：植物复合型；M3FX：Manα1 - 6(Manα1 - 3)(Xylβ1 - 2)Manβ1 - 4GlcNAcβ1 - 4(Fucα1 - 3)GlcNAc - PA；GN2M3FX：GlcNAcβl - 2Manα1 - 6(GlcNAcβl - 2Manα1 - 3)(Xylβ1 - 2)Manβ1 - 4GlcNAcβ1 - 4(Fucα1 - 3)GlcNAc - PA；(Le)1GN1M3FX：Galβ1 - 3(Fucα1 - 4)GlcNAc1 - 2 Manα1 - 6(GlcNAcβ1 - 2Manα1 - 3)(Xylβ1 - 2)Manβ1 - 4GlcNAcβ1 - 4(Fucα1 - 3)GlcNAc - PA或GlcNAc1 - 2Manα1 - 6(Galβ1 - 3(Fucα1 - 4)GlcNAc1 - 2Manα1 - 3)(Xylβ1 - 2)Manβ1 - 4GlcNAcβ1 - 4(Fucα1 - 3)GlcNAc - PA。
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