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RNA 测序分析揭示了三色苋叶片中甜菜碱代谢途径。

RNA-sequencing analysis reveals betalains metabolism in the leaf of Amaranthus tricolor L.

机构信息

Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, China.

Institut de la Recherche Interdisciplinaire de Toulouse, Toulouse, France.

出版信息

PLoS One. 2019 Apr 25;14(4):e0216001. doi: 10.1371/journal.pone.0216001. eCollection 2019.

DOI:10.1371/journal.pone.0216001
PMID:31022263
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6483260/
Abstract

Amaranth plants contain large amounts of betalains, including betaxanthins and betacyanins. Amaranthin is a betacyanin, and its molecular structure and associated metabolic pathway differ from those of betanin in beet plants. The chlorophyll, carotenoid, betalain, and flavonoid contents in amaranth leaves were analyzed. The abundance of betalain, betacyanin, and betaxanthin was 2-5-fold higher in the red leaf sectors than in the green leaf sectors. Moreover, a transcriptome database was constructed for the red and green sectors of amaranth leaves harvested from 30-day-old seedlings. 22 unigenes were selected to analyze the expression profiles in the two leaf sectors. The RNA-sequencing data indicated that many unigenes are involved in betalain metabolic pathways. The potential relationships between diverse metabolic pathways and betalain metabolism were analyzed. The validation of the expression of 22 selected unigenes in a qRT-PCR assay revealed the genes that were differentially expressed in the two leaf sectors. Betalains were biosynthesized in specific tissues of the red sectors of amaranth leaves. Almost all of the genes related to betalain metabolism were identified in the transcriptome database, and the expression profiles were different between the red sectors and green sectors in the leaf. Amaranth plants consist of diverse metabolic pathways, and the betalain metabolic pathway is linked to a group of other metabolic pathways.

摘要

苋菜植物含有大量甜菜碱，包括甜菜黄素和甜菜青素。苋菜红是一种甜菜青素，其分子结构和相关代谢途径与甜菜植物中的甜菜碱不同。分析了苋菜叶片中的叶绿素、类胡萝卜素、甜菜碱和类黄酮含量。在红叶部分，甜菜碱、甜菜青素和甜菜黄素的丰度比绿叶部分高 2-5 倍。此外，还构建了苋菜叶片红、绿两部分的转录组数据库。从 30 日龄幼苗收获的苋菜叶片中选择了 22 个基因进行分析，以分析两个叶片区域的表达谱。RNA-seq 数据表明，许多基因参与甜菜碱代谢途径。分析了不同代谢途径与甜菜碱代谢之间的潜在关系。在 qRT-PCR 测定中对 22 个选定的基因表达进行验证，发现了在两个叶片区域中差异表达的基因。甜菜碱在苋菜叶片的红色部分的特定组织中生物合成。在转录组数据库中几乎鉴定出与甜菜碱代谢相关的所有基因，并且叶片中的红色部分和绿色部分的表达谱不同。苋菜植物包含多种代谢途径，甜菜碱代谢途径与一组其他代谢途径相关联。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6483260/f38c917e0123/pone.0216001.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6483260/60d9ee71989c/pone.0216001.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6483260/1a58dd3024fc/pone.0216001.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6483260/75e5be810f69/pone.0216001.g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6483260/df78be31352d/pone.0216001.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6483260/18bbf1addc12/pone.0216001.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6483260/e5cc6090ad3c/pone.0216001.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6483260/969279e4915b/pone.0216001.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6483260/24e3209e5d1c/pone.0216001.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6483260/f38c917e0123/pone.0216001.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6483260/60d9ee71989c/pone.0216001.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6483260/1a58dd3024fc/pone.0216001.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6483260/75e5be810f69/pone.0216001.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6483260/b414cdbf4f48/pone.0216001.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6483260/df78be31352d/pone.0216001.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6483260/18bbf1addc12/pone.0216001.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6483260/e5cc6090ad3c/pone.0216001.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6483260/969279e4915b/pone.0216001.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6483260/24e3209e5d1c/pone.0216001.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6483260/f38c917e0123/pone.0216001.g010.jpg

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