Herold K C, Lancki D W, Dunn D E, Arai K, Fitch F W
Eur J Immunol. 1986 Dec;16(12):1533-8. doi: 10.1002/eji.1830161211.
To study the regulation of lymphokine production by T lymphocytes, we have characterized the activation of lymphokine genes in T cells by measuring the levels of lymphokine mRNA in cloned murine T lymphocytes after stimulation. Lymphokine mRNA was not detected in cells taken after seven days of maintenance culture. Following stimulation of T helper lymphocytes L2 and AD9.1 with concanavalin A, lymphokine mRNA appeared, reached peak levels and disappeared over a 43-h time period. A single stimulation event resulted in the induction of mRNA for interleukin 2 (IL 2), IL 3 and interferon gamma. Maximal mRNA levels were generally found at 6 h in the T helper lymphocytes, but could occur as late as 18 h. The lymphokine genes were expressed coordinately; however, in these cloned cells, IL 2 mRNA levels appeared to be lower than the other two mRNAs. Lymphokine titers in the supernatant fluids paralleled the appearance of mRNA but IL 2 titers began to fall after 12 h probably because of utilization of this lymphokine by the activated cells. In the cytolytic T lymphocyte, L3, qualitatively similar kinetics were found after stimulation by lectin or a clonotypic antibody with peak mRNA levels occurring later (18 h) with the antibody. These studies indicate a single stimulating event activates the lymphokine genes of T cells in a coordinate manner; the appearance of the lymphokines in supernatant fluids represents de novo synthesis of these proteins but the levels of lymphokines measured in supernatant fluids reflects both production and utilization rates, and exposure to IL 2 at the time of stimulation is not essential for the production of other lymphokines.
为了研究T淋巴细胞对淋巴因子产生的调控，我们通过测量克隆的小鼠T淋巴细胞在刺激后淋巴因子mRNA的水平，对T细胞中淋巴因子基因的激活进行了表征。在维持培养7天后取出的细胞中未检测到淋巴因子mRNA。在用伴刀豆球蛋白A刺激辅助性T淋巴细胞L2和AD9.1后，淋巴因子mRNA出现，在43小时的时间段内达到峰值水平并消失。单次刺激事件导致白细胞介素2（IL-2）、IL-3和干扰素γ的mRNA诱导。在辅助性T淋巴细胞中，最大mRNA水平通常在6小时时出现，但也可能在18小时时才出现。淋巴因子基因是协同表达的；然而，在这些克隆细胞中，IL-2 mRNA水平似乎低于其他两种mRNA。上清液中的淋巴因子滴度与mRNA的出现情况平行，但IL-2滴度在12小时后开始下降，可能是因为活化细胞利用了这种淋巴因子。在细胞毒性T淋巴细胞L3中，用凝集素或克隆型抗体刺激后发现了定性相似的动力学，抗体刺激后mRNA峰值水平出现得较晚（18小时）。这些研究表明，单次刺激事件以协同方式激活T细胞的淋巴因子基因；上清液中淋巴因子的出现代表了这些蛋白质的从头合成，但上清液中测量的淋巴因子水平反映了产生和利用率，并且在刺激时暴露于IL-2对于其他淋巴因子的产生不是必需的。
