Soll D R, Mitchell L, Kraft B, Alexander S, Finney R, Varnum-Finney B
Dev Biol. 1987 Mar;120(1):25-37. doi: 10.1016/0012-1606(87)90100-x.
The preaggregative period of Dictyostelium discoideum is composed of two sequential rate-limiting components. The timing mutant FM-1 exhibits a decrease in the length of the preaggregative period and the interval between the maxifinger and early culminate II stage. In contrast, it is normal in all aspects of growth, in the sequence of morphogenetic stages, in spore formation, in the capacity to rapidly recapitulate morphogenesis, and in the erasure event and subsequent program of dedifferentiation. By the reciprocal shift experiment, it is demonstrated that FM-1 is completely missing the first of the two rate-limiting components comprising the preaggregative period. The FM-1 mutation is heritable and behaves as a single mutation mapping to linkage group II. However, the FM-1 variant switches at relatively high frequency to several other timing phenotypes with longer preaggregative periods which in turn switch at high frequency. The FM-1 phenotype is considered in terms of timing regulation, and the process of high frequency switching between timing phenotypes is compared to other newly discovered switching systems.
盘基网柄菌的聚集前期由两个连续的限速成分组成。定时突变体FM - 1在聚集前期的时长以及最大指状期和早期发育成熟II期之间的间隔上有所缩短。相比之下，它在生长的各个方面、形态发生阶段的顺序、孢子形成、快速重现形态发生的能力以及去分化事件和随后的去分化程序方面都是正常的。通过相互转换实验表明，FM - 1完全缺失构成聚集前期的两个限速成分中的第一个。FM - 1突变是可遗传的，表现为一个映射到连锁群II的单基因突变。然而，FM - 1变体以相对较高的频率转换为其他几种具有较长聚集前期的定时表型，而这些表型又会高频转换。从定时调控的角度考虑FM - 1表型，并将定时表型之间的高频转换过程与其他新发现的转换系统进行比较。
