College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin Key Laboratory of Food Biotechnology, Tianjin, 300134, China.
College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin Key Laboratory of Food Biotechnology, Tianjin, 300134, China.
J Ethnopharmacol. 2019 Jul 15;239:111886. doi: 10.1016/j.jep.2019.111886. Epub 2019 Apr 23.
Sour Jujube seed from Ziziphus jujuba Mill. var. Spinosa (Bunge) Hu ex H. F. Chow is a traditional Chinese herb. It was demonstrated with significant activities in anti-depression and antioxidant by numerous pharmacological studies. Flavonoids is one of the main constituents in sour Jujube seed.
The aim of this study was to propose a green ultrasound-assisted extraction (UAE) process of flavonoids from sour Jujube seed.
The extraction parameters were investigated and optimized using single factor experiments, Plackett-Burman design (PBD) and response surface methodology (RSM). Moreover, a comparative analysis between ultrasound-assisted extraction and heat reflux extraction was performed to verify the ameliorating effects of ultrasound-assisted extraction on the flavonoids yield, the composition, antioxidant capacities in vitro and ROS scavenging capacity in PC12 cells. Meanwhile, the effects of flavonoids extract (FE) on Aβ transgenic Caenorhabditis elegans (GMC101) behavior were investigated.
The optimal extracting conditions of total flavonoids were as follows: ethanol concentration 70.60 (v/v%), liquid-solid ratio 15.02:1 mL/g, ultrasonic power 404 W, extraction time 60.03 min. The highest extraction yield was 1.59%. When compared to Heat reflux extraction (HRE) that only has gained a yield of 1.356%. Approximately, the UAE method was able to increase the yield by 17.11%. Moreover, FE extracted by UAE displayed larger capacity of scavenging ABTS, DPPH, superoxide, and hydroxyl radicals and reducing the level of ROS accumulation in PC12 cells, suggesting the biological functions of these compounds could be also better protected under UAE conditions. Furthermore, FE could also increase the chemotaxis and heat stress resistance ability, delay the paralysis and extend the lifespan of Caenorhabditis elegans.
UAE is a green and efficient technique for the preparation of flavonoids from sour Jujube seed. The flavonoids extract can reduce Aβ-induced toxicity in Caenorhabditis elegans.
酸枣仁（Ziziphus jujuba Mill. var. Spinosa（Bunge）Hu ex H. F. Chow）是一种传统的中国草药。大量药理学研究表明，它具有抗抑郁和抗氧化作用。黄酮类化合物是酸枣仁的主要成分之一。
本研究旨在提出一种绿色超声辅助提取（UAE）法从酸枣仁中提取黄酮类化合物。
采用单因素实验、Plackett-Burman 设计（PBD）和响应面法（RSM）对提取参数进行了考察和优化。此外，还对超声辅助提取与热回流提取进行了比较分析，以验证超声辅助提取对黄酮类化合物产率、组成、体外抗氧化能力和 PC12 细胞中 ROS 清除能力的改善作用。同时，还研究了黄酮类化合物提取物（FE）对 Aβ 转基因秀丽隐杆线虫（GMC101）行为的影响。
总黄酮的最佳提取条件为：乙醇浓度 70.60（v/v%），液固比 15.02:1 mL/g，超声功率 404 W，提取时间 60.03 min。最高提取率为 1.59%。与仅获得 1.356%产率的热回流提取（HRE）相比，UAE 法的提取率提高了 17.11%左右。此外，UAE 提取的 FE 具有更大的 ABTS、DPPH、超氧阴离子和羟基自由基清除能力，能降低 PC12 细胞中 ROS 积累水平，表明这些化合物的生物功能在 UAE 条件下也能得到更好的保护。此外，FE 还能提高秀丽隐杆线虫的趋化性和耐热应激能力，延缓其麻痹，延长其寿命。
UAE 是一种从酸枣仁中制备黄酮类化合物的绿色、高效技术。黄酮类化合物提取物能减轻 Aβ 诱导的秀丽隐杆线虫毒性。
