药物性牙龈过度增生的分子机制：氨氯地平与牙龈成纤维细胞的体外研究。
Molecular Aspects of Drug-Induced Gingival Overgrowth: An In Vitro Study on Amlodipine and Gingival Fibroblasts.
机构信息
Department of Medicine and Surgery, Centre of Neuroscience of Milan, University of Milano-Bicocca, 20126 Milan, Italy.
Multidisciplinary Department of Medical and Dental Specialties, University of Campania- Luigi Vanvitelli, 80138 Naples, Italy.
出版信息
Int J Mol Sci. 2019 Apr 25;20(8):2047. doi: 10.3390/ijms20082047.
UNLABELLED
Gingival overgrowth is a serious side effect that accompanies the use of amlodipine. Several conflicting theories have been proposed to explain the fibroblast's function in gingival overgrowth. To determine whether amlodipine alters the fibrotic response, we investigated its effects on treated gingival fibroblast gene expression as compared with untreated cells.
MATERIALS AND METHODS
Fibroblasts from ATCC Cell Lines were incubated with amlodipine. The gene expression levels of 12 genes belonging to the "Extracellular Matrix and Adhesion Molecules" pathway was investigated in treated fibroblasts cell culture, as compared with untreated cells, by real time PCR.
RESULTS
Most of the significant genes were up-regulated. (, , , , , , , ) except for , , , and , which were down-regulated.
CONCLUSION
These results seem to demonstrate that amlodipine has an effect on the extracellular matrix of gingival fibroblast. In the future, it would be interesting to understand the possible effect of the drug on fibroblasts of patients with amlodipine-induced gingival hyperplasia.
未加标签
牙龈增生是伴随氨氯地平使用出现的严重副作用。为解释成纤维细胞在牙龈增生中的功能，提出了几种相互矛盾的理论。为了确定氨氯地平是否改变纤维增生反应，我们研究了与未处理细胞相比，其对处理的牙龈成纤维细胞基因表达的影响。
材料与方法
从 ATCC 细胞系中分离出成纤维细胞，用氨氯地平孵育。通过实时 PCR 研究处理的成纤维细胞培养物中 12 个属于“细胞外基质和黏附分子”途径的基因的表达水平与未处理细胞相比的变化。
结果
大多数显著上调的基因（、、、、、、、、），下调的基因除外（、、、、、、）。
结论
这些结果似乎表明氨氯地平对牙龈成纤维细胞的细胞外基质有影响。将来，了解该药物对氨氯地平诱导的牙龈增生患者的成纤维细胞的可能影响将很有趣。
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