Yin Minzhen, Yang Mei, Chu Shanshan, Li Renqing, Zhao Yujiao, Peng Huasheng, Zhan Zhilai, Sun Hai Feng
Anhui University of Chinese Medicine, Pharmaceutical School, Hefei Anhui 230012, People's Republic of China.
China Academy of Chinese Medical Sciences, State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Beijing 100700, People's Republic of China.
J AOAC Int. 2019 May 1;102(3):734-740. doi: 10.5740/jaoacint.18-0308.
is a famous Chinese medicinal material whose producing area is Hunyuan, Shanxi. produced in Hunyuan, Shanxi, were divided into several different specifications and grades according to the diameters and different positions of root system. This article investigates the quantitative characteristics of chemical compositions in different specifications and grades of var. roots, aiming to elucidate the correlation between specifications and/or grades and chemical compositions in Based on the field investigation, samples of collected from Hunyuan, Shanxi, were divided into different specifications and grades. The content of seven flavonoids and five saponins in var. roots of different specifications and grades were determined simultaneously by HPLC-diode-array detection-evaporative light-scattering detection (HPLC-DAD-ELSD). was processed by traditional methods, and its commercial specification was classified by different parts of the root system, such as , , , , , and . The total content of seven flavonoids and five saponins in were lower. The total content of seven flavonoids in was much higher, while that of five saponins was much lower. The total content of seven flavonoids in lateral roots or fibrous roots were higher, and that of five saponins was lower, such as and According to the root diameters, was classified to special grade, grade I, grade II, grade III, grade IV, or grade V. Among six grades of the total content of seven flavonoids in grade III, grade IV, and grade V were lower, while the total content of five saponins in them were much higher. There is an obvious difference on the distribution pattern of contents of seven flavonoids and five saponins in of different specifications and grades, which provide a certain scientific basis for the quality evaluation of . The content of seven flavonoids and five saponins in were determined by HPLC-DAD-ELSD. The relationship between the commercial specification grades and chemical components of Dao-di herbs var. ( ) from Hunyuan, Shanxi were revealed, which provided a chemical basis for the classification of commercial specification grades of dao-herbs var. ( ) from Hunyuan, Shanxi.
是一种著名的中药材，其产地为山西浑源。产于山西浑源的[药材名称]根据根系直径和不同位置被分为几种不同的规格和等级。本文研究了[药材名称]变种不同规格和等级根系中化学成分的定量特征，旨在阐明[药材名称]变种规格和/或等级与化学成分之间的相关性。基于实地调查，从山西浑源采集的[药材名称]样本被分为不同的规格和等级。采用高效液相色谱 - 二极管阵列检测 - 蒸发光散射检测法（HPLC - DAD - ELSD）同时测定不同规格和等级的[药材名称]变种根系中七种黄酮类化合物和五种皂苷的含量。[药材名称]经传统方法加工，其商业规格根据根系的不同部位分类，如[具体部位1]、[具体部位2]、[具体部位3]、[具体部位4]、[具体部位5]和[具体部位6]。[某一规格或等级]中七种黄酮类化合物和五种皂苷的总含量较低。[另一规格或等级]中七种黄酮类化合物的总含量较高，而五种皂苷的总含量较低。侧根或须根中七种黄酮类化合物的总含量较高，而五种皂苷的总含量较低，如[具体例子1]和[具体例子2]。根据根直径，[药材名称]被分为特级、一级、二级、三级、四级或五级。在[药材名称]的六个等级中，三级、四级和五级中七种黄酮类化合物的总含量较低，而其中五种皂苷的总含量较高。不同规格和等级的[药材名称]中七种黄酮类化合物和五种皂苷含量的分布模式存在明显差异，这为[药材名称]的质量评价提供了一定的科学依据。采用HPLC - DAD - ELSD法测定了[药材名称]中七种黄酮类化合物和五种皂苷的含量。揭示了山西浑源道地药材[药材名称]变种（[具体学名]）商业规格等级与化学成分之间的关系，为山西浑源道地药材[药材名称]变种（[具体学名]）商业规格等级的划分提供了化学依据。
