Ibrahim F A, Elmansi H M, El Abass S A
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, 35516 Mansoura, Egypt.
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, 35516 Mansoura, Egypt.
Ann Pharm Fr. 2019 Jul;77(4):302-312. doi: 10.1016/j.pharma.2019.02.005. Epub 2019 Apr 23.
Timolol is a non-cardioselective beta blocker and has different combined ophthalmic dosage forms for treatment of glaucoma. This research introduce an HPLC method for the separation of three drugs used in combination with timolol simultaneously by applying isocratic mobile phase system in a single run and the same detection wavelength with short time. The drugs included in the separation procedures are; dorzolamide, brinzolamide, and brimonidine. The HPLC method was carried out through a single mobile phase system, which contains acetonitrile: 0.05M phosphate buffer at the ratio of 30:70, respectively at pH 3.5 and wavelength of 220nm. The method, regarding its simplicity allows determination of the studied drugs simultaneously using single run in about 8minutes. The method was rectilinear in the ranges of concentration: 1.25-25μg/mL for timolol, 4-80μg/mL for dorzolamide, 5-50μg/mL for brinzolamide and 2-20μg/mL for brimonidine. Different factors affecting the separation are thoroughly studied. The developed method was validated based on the official guidelines and the results were compared statistically with previously published methods and showed non-significant difference.
噻吗洛尔是一种非选择性β受体阻滞剂，有不同的眼科联合剂型用于治疗青光眼。本研究介绍了一种高效液相色谱（HPLC）方法，通过在单次运行中应用等度流动相系统和相同的检测波长，在短时间内同时分离与噻吗洛尔联合使用的三种药物。分离程序中包含的药物有：多佐胺、布林佐胺和溴莫尼定。HPLC方法通过单一流动相系统进行，该系统包含乙腈与0.05M磷酸盐缓冲液，比例分别为30:70，pH值为3.5，波长为220nm。该方法操作简单，可在约8分钟内单次运行同时测定所研究的药物。该方法在以下浓度范围内呈线性：噻吗洛尔为1.25 - 25μg/mL，多佐胺为4 - 80μg/mL，布林佐胺为5 - 50μg/mL，溴莫尼定为2 - 20μg/mL。对影响分离的不同因素进行了深入研究。所开发的方法根据官方指南进行了验证，结果与先前发表的方法进行了统计学比较，显示无显著差异。
