一种简单且易于复制的大鼠可逆性梗阻性黄疸模型。
A Simple and Easily Reproducible Model of Reversible Obstructive Jaundice in Rats.
作者信息
Hiratani Seigo, Mori Ryutaro, Ota Yohei, Matsuyama Ryusei, Kumamoto Takafumi, Nagashima Yoji, Morioka Daisuke, Endo Itaru
机构信息
Department of Gastroenterological Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan.
Department of Gastroenterological Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan
出版信息
In Vivo. 2019 May-Jun;33(3):699-706. doi: 10.21873/invivo.11528.
BACKGROUND/AIM: Cholangiocarcinoma and pancreatic carcinoma are major malignancies that cause obstructive jaundice (OJ). This study aimed to develop a simple and easily reproducible rat model of reversible OJ (ROJ).
MATERIALS AND METHODS
OJ was induced by clamping the common bile duct (CBD) using a U-shaped titanium hemoclip and its base was attached by ligation using 2-cm long 4-0 polypropylene suture. An anti-adhesive sheet was placed around the CBD. OJ was mitigated by pulling the suture to remove the clip under laparotomy 3 days later. Serum chemistry and liver histopathology were compared between the ROJ group and sham surgery (SH) groups.
RESULTS
Three days after inducing OJ, serum total bilirubin, aspartate aminotransferase, and alanine aminotransferase were remarkably elevated in the ROJ group and thereafter reduced significantly after mitigating OJ. Similar findings were confirmed by histopathology.
CONCLUSION
Our rat model of reversible OJ was considered simple and easily reproducible.
背景/目的：胆管癌和胰腺癌是导致梗阻性黄疸（OJ）的主要恶性肿瘤。本研究旨在建立一种简单且易于重复的可逆性梗阻性黄疸（ROJ）大鼠模型。
材料与方法
使用U形钛夹夹闭胆总管（CBD）诱导OJ，并用2厘米长的4-0聚丙烯缝线结扎钛夹基部。在CBD周围放置防粘连片。3天后通过剖腹手术拉动缝线移除夹子来减轻OJ。比较ROJ组和假手术（SH）组的血清生化指标和肝脏组织病理学。
结果
诱导OJ后3天，ROJ组血清总胆红素、天冬氨酸转氨酶和丙氨酸转氨酶显著升高，减轻OJ后显著降低。组织病理学证实了类似的结果。
结论
我们的可逆性OJ大鼠模型被认为简单且易于重复。
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