Kunowska Natalia
Wellcome Sanger Institute, Wellcome Genome Campus, Cambridge, UK.
Methods Mol Biol. 2019;1979:235-250. doi: 10.1007/978-1-4939-9240-9_15.
DNA methylation at cytosine is a major epigenetic mark, heavily implicated in controlling key cellular processes such as development and differentiation, cellular memory, or carcinogenesis. Bisulfite treatment in conjunction with next generation sequencing has been a powerful tool for studying this modification in a quantitative manner in the context of the whole genome and with a single nucleotide resolution. This chapter describes a protocol for bisulfite sequencing adapted to a single-cell format that allows for capturing the methylation signal from up to 50% CpG nucleotides in each cell.
胞嘧啶的DNA甲基化是一种主要的表观遗传标记，在控制关键细胞过程（如发育与分化、细胞记忆或致癌作用）中发挥着重要作用。亚硫酸氢盐处理结合新一代测序技术，已成为在全基因组范围内以单核苷酸分辨率定量研究这种修饰的强大工具。本章描述了一种适用于单细胞形式的亚硫酸氢盐测序方案，该方案能够从每个细胞中捕获高达50%的CpG核苷酸的甲基化信号。
