Zakharian Eleonora
Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine, Peoria, IL, USA.
Methods Mol Biol. 2019;1987:143-166. doi: 10.1007/978-1-4939-9446-5_10.
The family of the transient receptor potential (TRP) proteins presents a diverse group of polymodal ion channels intertwined in the regulation of various physiological processes. Currently, TRP channels are well established in temperature-sensation, thermoregulation, pain sensation, and mineral homeostasis. Furthermore, new evidence suggests that TRP channels are also implicated in hormonal signaling, where the channels are responsible for propagating hormone-induced signals along the neural circuitry and also regulating cellular processes of nonexcitable cells. Due to this wide assortment of actions, TRP channels have been attracting immense scientific interest in various fields.In this chapter, I describe incorporation and characterization of several TRP channels using an electrophysiological approach known as planar lipid bilayers. This technique features measurements of functional activities of ion channels in a well-defined reconstituted system. The priority of this electrophysiological approach is identifying intrinsic properties of ion channels, which is particularly valuable in appreciating intrinsic temperature sensitivity concerning thermo-TRP channels, but also direct mechanisms of channels agonists, antagonists, cofactors, and other modifiers.
瞬时受体电位（TRP）蛋白家族是一类多样的多模态离子通道，它们在各种生理过程的调节中相互交织。目前，TRP通道在温度感知、体温调节、痛觉和矿物质稳态方面已得到充分证实。此外，新证据表明，TRP通道也参与激素信号传导，这些通道负责沿着神经回路传递激素诱导的信号，并调节非兴奋性细胞的细胞过程。由于其广泛的作用，TRP通道在各个领域都引起了极大的科学兴趣。在本章中，我将描述使用一种称为平面脂质双层的电生理方法对几种TRP通道进行整合和表征。该技术的特点是在一个明确的重组系统中测量离子通道的功能活性。这种电生理方法的重点是确定离子通道的内在特性，这在了解热TRP通道的内在温度敏感性方面特别有价值，同时也有助于了解通道激动剂、拮抗剂、辅因子和其他调节剂的直接作用机制。
