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从山羊乳腺中分离纯化细胞视黄醇结合蛋白(CRBP)。

Isolation and purification of cellular retinol binding protein (CRBP) from goat mammary gland.

作者信息

Goyal K C, Sharma Y K, Misra U K

出版信息

Life Sci. 1987 Mar 2;40(9):863-70. doi: 10.1016/0024-3205(87)90035-x.

DOI:10.1016/0024-3205(87)90035-x
PMID:3102874
Abstract

The cellular retinol binding protein (CRBP) from goat mammary gland has been isolated in pure form. The molecular weight of CRBP by gel filtration was 15,120 dalton. The 280/350 absorbance ratio of this protein was 1.20 and it gave a single precipitin line on immunodiffusion. The method is very simple and reproducible.

摘要

已从山羊乳腺中以纯形式分离出细胞视黄醇结合蛋白(CRBP)。通过凝胶过滤测得CRBP的分子量为15,120道尔顿。该蛋白的280/350吸光度比值为1.20,在免疫扩散中产生单一沉淀线。该方法非常简单且可重复。

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引用本文的文献

1
Secretion pattern of retinol-binding protein in blood of goats: effect of vitamin A, provitamin A and their dosing schedule.
Z Ernahrungswiss. 1989 Sep;28(3):225-30. doi: 10.1007/BF02023692.